BMRB Entry 11182

Title:
Solution structure of the PDZ domain of human KIAA0340 protein
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Inoue, K.; Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Inoue, K.; Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of human KIAA0340 protein"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040628-04

Data sets:
Data typeCount
13C chemical shifts512
15N chemical shifts117
1H chemical shifts833

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 121 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALTHRTRP
2   GLNPROSERLYSGLUGLYASPARGLEUILE
3   GLYARGVALILELEUASNLYSARGTHRTHR
4   METPROLYSASPSERGLYALALEULEUGLY
5   LEULYSVALVALGLYGLYLYSMETTHRASP
6   LEUGLYARGLEUGLYALAPHEILETHRLYS
7   VALLYSLYSGLYSERLEUALAASPVALVAL
8   GLYHISLEUARGALAGLYASPGLUVALLEU
9   GLUTRPASNGLYLYSPROLEUPROGLYALA
10   THRASNGLUGLUVALTYRASNILEILELEU
11   GLUSERLYSSERGLUPROGLNVALGLUILE
12   ILEVALSERARGPROSERGLYPROSERSER
13   GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.33 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAE22200 BAH11605 BAH14479
GB EMP34190 EPY76123 ETE71210
REF NP_001161881 XP_002690061 XP_003271153 XP_004044328 XP_004381426

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks