BMRB Entry 11333

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PDB ID: 2dkt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11333
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus" .

Assembly members:

Assembly members:
CHY domian, polymer, 143 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030421-68

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CHY domian: GSSGSSGGVRNLAQGPRGCE HYDRACLLKAPCCDKLYTCR LCHDTNEDHQLDRFKVKEVQ CINCEKLQHAQQTCEDCSTL FGEYYCSICHLFDKDKRQYH CESCGICRIGPKEDFFHCLK CNLCLTTNLRGKHKCIESGP SSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	573
15N chemical shifts	137
1H chemical shifts	903

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CHY domian	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2
4	ZINC ION no.3	2
5	ZINC ION no.4	2
6	ZINC ION no.5	2
7	ZINC ION no.6	2

Entities:

Entity 1, CHY domian 143 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

ARG

2

ASN

LEU

ALA

GLN

GLY

PRO

ARG

GLY

CYS

GLU

3

HIS

TYR

ASP

ARG

ALA

CYS

LEU

LYS

ALA

4

PRO

CYS

ASP

LYS

LEU

TYR

THR

CYS

ARG

5

LEU

CYS

HIS

ASP

THR

ASN

GLU

ASP

HIS

GLN

6

LEU

ASP

ARG

PHE

LYS

VAL

LYS

GLU

VAL

GLN

7

CYS

ILE

ASN

CYS

GLU

LYS

LEU

GLN

HIS

ALA

8

GLN

THR

CYS

GLU

ASP

CYS

SER

THR

LEU

9

PHE

GLY

GLU

TYR

CYS

SER

ILE

CYS

HIS

10

LEU

PHE

ASP

LYS

ASP

LYS

ARG

GLN

TYR

HIS

11

CYS

GLU

SER

CYS

GLY

ILE

CYS

ARG

ILE

GLY

12

PRO

LYS

GLU

ASP

PHE

HIS

CYS

LEU

LYS

13

CYS

ASN

LEU

CYS

LEU

THR

ASN

LEU

ARG

14

GLY

LYS

HIS

LYS

CYS

ILE

GLU

SER

GLY

PRO

15

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: CHY domain, [U-13C; U-15N], 1.23 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz