BMRB Entry 11351

Title:
Solution structure of the NEUZ (NHR) domain in Neuralized from Drosophila melanogaster
Deposition date:
2010-09-07
Original release date:
2011-09-07
Authors:
He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Tarada, T.; Yokoyama, S.
Citation:

Citation: He, F.; Saito, K.; Kobayashi, N.; Harada, T.; Watanabe, S.; Kigawa, T.; Guntert, P.; Ohara, O.; Tanaka, A.; Unzai, S.; Muto, Y.; Yokoyama, S.. "Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway."  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
NEUZ(NHR) domain, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P061002-04

Data sets:
Data typeCount
13C chemical shifts733
15N chemical shifts173
1H chemical shifts1144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NEUZ(NHR) domain1

Entities:

Entity 1, NEUZ(NHR) domain 168 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLEUGLN
2   PHEHISSERVALHISGLYASPASNILEARG
3   ILESERARGASPGLYTHRLEUALAARGARG
4   PHEGLUSERPHECYSARGALAILETHRPHE
5   SERALAARGPROVALARGILEASNGLUARG
6   ILECYSVALLYSPHEALAGLUILESERASN
7   ASNTRPASNGLYGLYILEARGPHEGLYPHE
8   THRSERASNASPPROVALTHRLEUGLUGLY
9   THRLEUPROLYSTYRALACYSPROASPLEU
10   THRASNARGPROGLYPHETRPALALYSALA
11   LEUHISGLUGLNTYRCYSGLULYSASPASN
12   ILELEUTYRTYRTYRVALASNGLYALAGLY
13   ASPVALILETYRGLYILEASNASNGLUGLU
14   LYSGLYVALILELEUTHRGLYILEASPTHR
15   ARGSERLEULEUTRPTHRVALILEASPILE
16   TYRGLYASNCYSTHRGLYILEGLUPHELEU
17   ASPSERARGILETYRMETTYRGLN

Samples:

sample_1: NEUZ(NHR) domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks