BMRB Entry 15191

Title:
backbone and sidechain order parameters for CaM-nNOSp
Deposition date:
2007-03-19
Original release date:
2007-10-05
Authors:
Valentine, Kathleen; Wand, A; Frederick, Kendra; Marlow, Michael
Citation:

Citation: Frederick, Kendra; Marlow, Michael; Valentine, Kathleen; Wand, A. "Conformational entropy in molecular recognition by proteins"  Nature 448, 325-329 (2007).
PubMed: 17637663

Assembly members:

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
nNOSp, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data typeCount
order parameters194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2nNOSp2

Entities:

Entity 1, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, nNOSp 23 residues - Formula weight is not available

1   LYSARGARGALAILEGLYPHELYSLYSLEU
2   ALAGLUALAVALLYSPHESERALALYSLEU
3   METGLYGLN

Samples:

sample_1: Calmodulin, [U-100% 15N], 1.0 mM; nNOSp 1.2 mM; potassium chloride 100 mM; imidazole 20 mM; calcium chloride 6 mM; sodium azide 3 mM

sample_2: Calmodulin, [U-100% 13C; U-100% 15N; 55% 2H], 1.0 mM; nNOSp 1.2 mM; potassium chloride 100 mM; imidazole 20 mM; calcium chloride 6 mM; sodium azide 3 mM

sample_conditions_1: ionic strength: 112 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

FELIX vIn house modified, Hare software - peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB 3415119