BMRB Entry 15505

Title:
CypB
Deposition date:
2007-10-04
Original release date:
2008-06-26
Authors:
Landrieu, Isabelle
Citation:

Citation: Hanoulle, Xavier; Melchior, Aurelie; Sibille, Nathalie; Parent, B.; Denys, Agnes; Wieruszeski, Jean-Michel; Horvath, Dragos; Allain, Fabrice; Lippens, Guy; Landrieu, Isabelle. "Structural and functional characterization of the interaction between cyclophilin B and a heparin-derived oligosaccharide."  J. Biol. Chem. 282, 34148-34158 (2007).
PubMed: 17855358

Assembly members:

Assembly members:
CypB, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts175
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CypB1

Entities:

Entity 1, CypB 184 residues - Formula weight is not available

1   ALAASPGLULYSLYSLYSGLYPROLYSVAL
2   THRVALLYSVALTYRPHEASPLEUARGILE
3   GLYASPGLUASPVALGLYARGVALILEPHE
4   GLYLEUPHEGLYLYSTHRVALPROLYSTHR
5   VALASPASNPHEVALALALEUALATHRGLY
6   GLULYSGLYPHEGLYTYRLYSASNSERLYS
7   PHEHISARGVALILELYSASPPHEMETILE
8   GLNGLYGLYASPPHETHRARGGLYASPGLY
9   THRGLYGLYLYSSERILETYRGLYGLUARG
10   PHEPROASPGLUASNPHELYSLEULYSHIS
11   TYRGLYPROGLYTRPVALSERMETALAASN
12   ALAGLYLYSASPTHRASNGLYSERGLNPHE
13   PHEILETHRTHRVALLYSTHRALATRPLEU
14   ASPGLYLYSHISVALVALPHEGLYLYSVAL
15   LEUGLUGLYMETGLUVALVALARGLYSVAL
16   GLUSERTHRLYSTHRASPSERARGASPLYS
17   PROLEULYSASPVALILEILEALAASPCYS
18   GLYLYSILEGLUVALGLULYSPROPHEALA
19   ILEALALYSGLU

Samples:

sample_1: CypB, [U-99% 15N], 0.5 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 40 mM; EDTA 1 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_2: CypB, [U-98% 13C; U-98% 15N], 0.5 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 40 mM; EDTA 1 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_3: CypB, [U-15N, U-2H], 0.3 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 40 mM; EDTA 1 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_4: CypB, [U-15N; U-2H], 0.3 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 40 mM; EDTA 1 mM; DTT 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.04 M; pH: 6.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 25338
PDB
DBJ BAF83838 BAI46658 BAK96182
EMBL CAG33110
GB AAA35733 AAA36601 AAA52150 AAH01125 AAH08848
REF NP_000933 NP_001253241 NP_001265776 XP_002718189 XP_002753266
SP P23284
AlphaFold P23284

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks