BMRB Entry 15520

Title:
SOLUTION STRUCTURE OF ENGRAILED HOMEODOMAIN L16A MUTANT
Deposition date:
2007-10-12
Original release date:
2007-10-16
Authors:
Religa, Tomasz; Markson, Joseph; Mayor, Ugo; Freund, Stefan; Fersht, Alan
Citation:

Citation: Religa, Tomasz; Markson, Joseph; Mayor, Ugo; Freund, Stefan; Fersht, Alan. "Solution Structure of a Protein Denatured State and Folding Intermediate."  Nature 437, 1053-1056 (2005).
PubMed: 16222301

Assembly members:

Assembly members:
SEGMENTATION_POLARITY_HOMEOBOX_PROTEIN_ENGRAILED, polymer, 61 residues, 7337.3 Da.

Natural source:

Natural source:   Common Name: FRUIT FLY   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: DROSOPHILA MELANOGASTER

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PRSET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SEGMENTATION_POLARITY_HOMEOBOX_PROTEIN_ENGRAILED: GDEKRPRTAFSSEQLARAKR EFNENRYLTERRRQQLSSEL GLNEAQIKIWFQNKRAKIKK S

Data sets:
Data typeCount
13C chemical shifts163
15N chemical shifts58
1H chemical shifts454
coupling constants51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED1

Entities:

Entity 1, SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED 61 residues - 7337.3 Da.

1   GLYASPGLULYSARGPROARGTHRALAPHE
2   SERSERGLUGLNLEUALAARGALALYSARG
3   GLUPHEASNGLUASNARGTYRLEUTHRGLU
4   ARGARGARGGLNGLNLEUSERSERGLULEU
5   GLYLEUASNGLUALAGLNILELYSILETRP
6   PHEGLNASNLYSARGALALYSILELYSLYS
7   SER

Samples:

15N: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

15N_PheTyr-H: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N; U-2H] [U-15N; U-1H]-Phe,Trp, 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

15N_U-D: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-15N; U-2H], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

13C15N: SEGMENTATION POLARITY HOMEOBOX PROTEIN ENGRAILED, [U-13C; U-15N], 500 uM; D-ACETATE 50 mM; NACL 100 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 150 mM; pH: 5.7; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D NOESY15Nisotropicsample_conditions_1
3D_15N-SEPARATED_ NOESY15N_PheTyr-Hisotropicsample_conditions_1
HSQC-NOESY-HSQC, 600MS MIXING TIME15N_U-Disotropicsample_conditions_1
3D_13C-SEPARATED_ NOESY13C15Nisotropicsample_conditions_1
3D HNHA15Nisotropicsample_conditions_1

Software:

NMRPIPE 2004-04-12 v2004-04-12, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - structure solution

SPARKY v3.106, Goddard - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • BRUKER DRX 800 MHz
  • BRUKER DRX 500 MHz

Related Database Links:

PDB
BMRB 15536
DBJ BAN82729 BAN82730 BAN82731
EMBL CAA28436
GB AAA65478 AAF58639 AAL39593 AAM68711 ACL84189
REF NP_523700 NP_725059 XP_001958778 XP_001976053 XP_002006437
SP P02836 P09145
AlphaFold P09145 P02836

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks