BMRB Entry 15602

Title:
1H, 13C and 15N chemical shift assignments of human PARP-1 domain C
Deposition date:
2007-12-20
Original release date:
2008-05-05
Authors:
Hoffman, David; Tao, Zhihua; Liu, Hung-wen
Citation:

Citation: Tao, Zhihua; Gao, Peng; Hoffman, David; Liu, Hung-wen. "Domain C of Human Poly(ADP-ribose) Polymerase-1 is Important for Enzyme Activity and Contains a Novel Zinc-ribbon Motif"  Biochemistry 47, 5804-5813 (2008).
PubMed: 18452307

Assembly members:

Assembly members:
domain_C_of_human_PARP-1, polymer, 144 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMALE

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts141
1H chemical shifts847

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1domain C polypeptide1
2zinc2

Entities:

Entity 1, domain C polypeptide 144 residues - Formula weight is not available

1   GLYHISMETLYSLEUGLULYSALALEULYS
2   ALAGLNASNASPLEUILETRPASNILELYS
3   ASPGLULEULYSLYSVALCYSSERTHRASN
4   ASPLEULYSGLULEULEUILEPHEASNLYS
5   GLNGLNVALPROSERGLYGLUSERALAILE
6   LEUASPARGVALALAASPGLYMETVALPHE
7   GLYALALEULEUPROCYSGLUGLUCYSSER
8   GLYGLNLEUVALPHELYSSERASPALATYR
9   TYRCYSTHRGLYASPVALTHRALATRPTHR
10   LYSCYSMETVALLYSTHRGLNTHRPROASN
11   ARGLYSGLUTRPVALTHRPROLYSGLUPHE
12   ARGGLUILESERTYRLEULYSLYSLEULYS
13   VALLYSLYSGLNASPARGILEPHEPROPRO
14   GLUTHRSERALASERVALALAALATHRPRO
15   PROPROSERTHR

Entity 2, zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: domain C of human PARP-1, [U-13C; U-15N], 1 mM; sodium phosphate buffer 10 mM; NaCl 250 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 260 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HACACBCOsample_1isotropicsample_conditions_1
3D TOCSY-HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - display NMR spectrum

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAG10250 BAG35260 BAG64403
GB AAA51663 AAA60137 AAA60155 AAB59447 AAH37545
REF NP_001609 XP_001090628 XP_001090984 XP_002809428 XP_003357689
SP P09874
AlphaFold P09874

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks