BMRB Entry 15853

Name: Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR
Published: 2008-08-07

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15853

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR

Deposition date:

2008-07-02

Original release date:

2008-08-07

Authors:

Chill, Jordan; Louis, John; Delaglio, Frank; Bax, Ad

Citation:

Citation: Chill, Jordan; Louis, John; Delaglio, Frank; Bax, Ad. "Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR" Biochim. Biophys. Acta. 1768, 3260-3270 (2007).
PubMed: 17945182

Assembly members:

Assembly members:
KcsA, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Streptomyces coelicolor Taxonomy ID: 1902 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces coelicolor

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KcsA: LLGRHGSALHWRAAGAATVL LVIVLLAGSYLAVLAERGAP GAQLITYPRALWWSVETATT VGYGDLYPVTLWGRLVAVVV MVAGITSFGLVTAALATWFV GREQERRGHFVRHSEKAAEE AYTRTTRALHERFDRLERML DDNRR

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	833
15N chemical shifts	407
1H chemical shifts	407

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KcsA	1

Entities:

Entity 1, KcsA 145 residues - Formula weight is not available

The first residue with number 1 is in reality the 16th residue.

1

LEU

GLY

ARG

HIS

GLY

SER

ALA

LEU

HIS

2

TRP

ARG

ALA

GLY

ALA

THR

VAL

LEU

3

LEU

VAL

ILE

VAL

LEU

ALA

GLY

SER

TYR

4

LEU

ALA

VAL

LEU

ALA

GLU

ARG

GLY

ALA

PRO

5

GLY

ALA

GLN

LEU

ILE

THR

TYR

PRO

ARG

ALA

6

LEU

TRP

SER

VAL

GLU

THR

ALA

THR

7

VAL

GLY

TYR

GLY

ASP

LEU

TYR

PRO

VAL

THR

8

LEU

TRP

GLY

ARG

LEU

VAL

ALA

VAL

9

MET

VAL

ALA

GLY

ILE

THR

SER

PHE

GLY

LEU

10

VAL

THR

ALA

LEU

ALA

THR

TRP

PHE

VAL

11

GLY

ARG

GLU

GLN

GLU

ARG

GLY

HIS

PHE

12

VAL

ARG

HIS

SER

GLU

LYS

ALA

GLU

13

ALA

TYR

THR

ARG

THR

ARG

ALA

LEU

HIS

14

GLU

ARG

PHE

ASP

ARG

LEU

GLU

ARG

MET

LEU

15

ASP

ASN

ARG

Samples:

sample_1_for_TableS2: KcsA 0.5-0.6 mM; sodium formate 25 mM; D2O 7%

sample_2_for_TableS3: KcsA 0.5-0.6 mM; MES 25 mM; D2O 7%

sample_3_for_TableS4: KcsA 0.5-0.6 mM; TRIS 25 mM; D2O 7%

sample_conditions_1: pH: 4.2; pressure: 14.1 Torr; temperature: 323 K

sample_conditions_2: pH: 6; pressure: 14.1 Torr; temperature: 323 K

sample_conditions_3: pH: 8; pressure: 14.1 Torr; temperature: 323 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1_for_TableS2	isotropic	sample_conditions_1
3D HNCO	sample_2_for_TableS3	isotropic	sample_conditions_2
3D HNCO	sample_3_for_TableS4	isotropic	sample_conditions_3
3D HN(CA)CO	sample_1_for_TableS2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2_for_TableS3	isotropic	sample_conditions_2
3D HN(CA)CO	sample_3_for_TableS4	isotropic	sample_conditions_3
3D HNCA	sample_1_for_TableS2	isotropic	sample_conditions_1
3D HNCA	sample_2_for_TableS3	isotropic	sample_conditions_2
3D HNCA	sample_3_for_TableS4	isotropic	sample_conditions_3

Software:

NMRPipe/NMRDraw, NIH - data analysis

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1BL8 1F6G 1J95 1JVM 1K4C 1K4D 1R3I 1R3J 1R3K 1R3L 1S5H 1ZWI 2ATK 2BOB 2BOC 2DWD 2DWE 2HJF 2HVJ 2HVK 2IH1 2IH3 2ITC 2ITD 2JK5 2NLJ 2P7T 2QTO 2W0F 3EFF 3F5W 3F7V 3F7Y 3FB5 3FB6 3FB7 3FB8 3GB7 3HPL 3IGA 3OGC 3OR6 3OR7 3STL 3STZ 4LBE 4LCU 4MSW 4UUJ
EMBL	CAA86025 CAC16993
GB	AIJ11233 EFD64549 EOY52587 KKD16939
REF	NP_631700 WP_003971485 WP_016328171 WP_030864493
SP	P0A333 P0A334
AlphaFold	P0A334 P0A333