BMRB Entry 15855

Title:
NMR assignment of human Growth Arrest and DNA Damage alpha protein (Gadd45a)
Deposition date:
2008-07-03
Original release date:
2008-07-07
Authors:
Sanchez, Ricardo; Pantoja-Uceda, David; Torres, Daniel; Prieto, Jesus; Campos-Olivas, Ramon; Blanco, Francisco J.
Citation:

Citation: Sanchez, Ricardo; Pantoja-Uceda, David; Torres, Daniel; Prieto, Jesus; Campos-Olivas, Ramon; Blanco, Francisco. "NMR assignment and secondary structure of human growth arrest and DNA damage alpha protein (Gadd45 alpha)"  Biomol. NMR Assignments 2, 139-142 (2008).
PubMed: 19636889

Assembly members:

Assembly members:
Gadd45a, polymer, 168 residues, 18627 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET28

Data sets:
Data typeCount
13C chemical shifts627
15N chemical shifts153
1H chemical shifts990

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gadd45a1

Entities:

Entity 1, Gadd45a 168 residues - 18627 Da.

Residues from -2 to 0 (GPH) represent a non-native tag in N-terminus

1   GLYPROHISMETTHRLEUGLUGLUPHESER
2   ALAGLYGLUGLNLYSTHRGLUARGMETASP
3   LYSVALGLYASPALALEUGLUGLUVALLEU
4   SERLYSALALEUSERGLNARGTHRILETHR
5   VALGLYVALTYRGLUALAALALYSLEULEU
6   ASNVALASPPROASPASNVALVALLEUCYS
7   LEULEUALAALAASPGLUASPASPASPARG
8   ASPVALALALEUGLNILEHISPHETHRLEU
9   ILEGLNALAPHECYSCYSGLUASNASPILE
10   ASNILELEUARGVALSERASNPROGLYARG
11   LEUALAGLULEULEULEULEUGLUTHRASP
12   ALAGLYPROALAALASERGLUGLYALAGLU
13   GLNPROPROASPLEUHISCYSVALLEUVAL
14   THRASNPROHISSERSERGLNTRPLYSASP
15   PROALALEUSERGLNLEUILECYSPHECYS
16   ARGGLUSERARGTYRMETASPGLNTRPVAL
17   PROVALILEASNLEUPROGLUARG

Samples:

HGa_CN: Gadd45a, [U-99% 13C; U-99% 15N], 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_N: Gadd45a, [U-99% 15N], 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_D2O: Gadd45a 195 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT-d10 2 mM; EDTA-d12 2 mM; sodium azide 0.03%

HGa_15NLeu: Gadd45a, [U-15N]-Leu, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NVal: Gadd45a, [U-15N]-Val, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NIle: Gadd45a, [U-15N]-Ile, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NPhe_Tyr: Gadd45a, [U-15N]-Phe/Tyr, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.27 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCHGa_Nisotropicsample_conditions_1
2D 1H-13C HMQC AliphaticHGa_CNisotropicsample_conditions_1
3D HNCOHGa_CNisotropicsample_conditions_1
3D HNCAHGa_CNisotropicsample_conditions_1
3D HNCACBHGa_CNisotropicsample_conditions_1
3D CBCA(CO)NHHGa_CNisotropicsample_conditions_1
3D HN(CO)CAHGa_CNisotropicsample_conditions_1
3D HNHAHGa_Nisotropicsample_conditions_1
3D 1H-15N NOESYHGa_Nisotropicsample_conditions_1
2D 1H-1H NOESYHGa_D2Oisotropicsample_conditions_1
2D 1H-15N HSQCHGa_15NLeuisotropicsample_conditions_1
2D 1H-15N HSQCHGa_15NValisotropicsample_conditions_1
2D 1H-15N HSQCHGa_15NIleisotropicsample_conditions_1
2D 1H-15N HSQCHGa_15NPhe_Tyrisotropicsample_conditions_1
4D 1H-15N 1H13C NOESYHGa_CNisotropicsample_conditions_1
2D 1H-13C HMQC AromaticHGa_CNisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

NMRPipe v1.02, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance II 800 MHz
  • Bruker Avance II 900 MHz

Related Database Links:

PDB
DBJ BAD60923 BAG37487 BAG73211
EMBL CAL38317
GB AAA35863 AAA72045 AAH11757 AAI03199 AAM88884
REF NP_001029419 NP_001038064 NP_001248234 NP_001915 XP_002715979
SP P24522 Q3ZBN6 Q60GI5
TPG DAA31278
AlphaFold P24522 Q60GI5 Q3ZBN6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks