BMRB Entry 15927

Title:
1H, 13C, 15N NMR resonance assignments of the actinoporin Sticholysin I
Deposition date:
2008-08-20
Original release date:
2008-11-17
Authors:
Castrillo, Ines; Santoro, Jorge; Bruix, Marta
Citation:

Citation: Castrillo, Ines; Alegre-Cebollada, Jorge; del Pozo, Alvaro Martinez; Gavilanes, Jose; Santoro, Jorge; Bruix, Marta. "1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I"  Biomol. NMR Assignments 3, 5-7 (2009).
PubMed: 19636934

Assembly members:

Assembly members:
StnI, polymer, 176 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Stichodactyla helianthus   Taxonomy ID: 6123   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Stichodactyla heliantus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE60

Data sets:
Data typeCount
13C chemical shifts559
15N chemical shifts184
1H chemical shifts1199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1StnI1

Entities:

Entity 1, StnI 176 residues - Formula weight is not available

1   SERGLULEUALAGLYTHRILEILEASPGLY
2   ALASERLEUTHRPHEGLUVALLEUASPLYS
3   VALLEUGLYGLULEUGLYLYSVALSERARG
4   LYSILEALAVALGLYILEASPASNGLUSER
5   GLYGLYTHRTRPTHRALALEUASNALATYR
6   PHEARGSERGLYTHRTHRASPVALILELEU
7   PROGLUVALVALPROASNTHRLYSALALEU
8   LEUTYRSERGLYARGLYSSERSERGLYPRO
9   VALALATHRGLYALAVALALAALAPHEALA
10   TYRTYRMETSERASNGLYASNTHRLEUGLY
11   VALMETPHESERVALPROPHEASPTYRASN
12   TRPTYRSERASNTRPTRPASPVALLYSILE
13   TYRPROGLYLYSARGARGALAASPGLNGLY
14   METTYRGLUASPMETTYRTYRGLYASNPRO
15   TYRARGGLYASPASNGLYTRPTYRGLNLYS
16   ASNLEUGLYTYRGLYLEUARGMETLYSGLY
17   ILEMETTHRSERALAGLYGLUALALYSMET
18   GLNILELYSILESERARG

Samples:

15N-13C_StnI: StnI, [U-13C; U-15N], 0.5 mM

1H_StnI: StnI 0.5 mM

sample_conditions_15N-13C_Stn1: pH: 4.0; pressure: 1 atm; temperature: 298 K

sample_conditions_1H_StnI: pH: 4.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
2D 1H-13C HSQC15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HNCACB15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D CBCA(CO)NH15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HN(CO)CA15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HNCA15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HCCH-TOCSY15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HNHA15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HBHA(CO)NH15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D HBHANH15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D 1H-15N NOESY15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
3D 1H-13C NOESY15N-13C_StnIisotropicsample_conditions_15N-13C_Stn1
2D 1H-1H TOCSY1H_StnIisotropicsample_conditions_1H_StnI
2D 1H-1H COSY1H_StnIisotropicsample_conditions_1H_StnI
2D 1H-1H NOESY1H_StnIisotropicsample_conditions_1H_StnI

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN v3.1, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AV800 800.2 MHz

Related Database Links:

PDB
EMBL CAC00651
SP P81662
AlphaFold P81662

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks