BMRB Entry 15954

Name: human eIF5A
Published: 2009-01-06

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15954

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human eIF5A

Deposition date:

2008-09-16

Original release date:

2009-01-06

Authors:

Jinqiao, Yuan; Nan, Jiang; Changwen, Jin; Xuemin, Zhang; Xianzhong, Yan

Citation:

Citation: Yuan, Jinqiao; Jiang, Nan; Jin, Changwen; Zhang, Xuemin; Yan, Xianzhong. "Backbone and sidechain 1H, 15N, and 13C assignments of the human eIF5A" Biomol. NMR Assignments 3, 25-28 (2009).
PubMed: 19636939

Assembly members:

Assembly members:
eIF5A, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF5A: MADDLDFETGDAGASATFPM QCSALRKNGFVVLKGRPCKI VEMSTSKTGKHGHAKVHLVG IDIFTGKKYEDICPSTHNMD VPNIKRNDFQLIGIQDGYLS LLQDSGEVREDLRLPEGDLG KEIEQKYDCGEEILITVLSA MTEEAAVAIKAMA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	454
15N chemical shifts	155
1H chemical shifts	997

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eIF5A	1

Entities:

Entity 1, eIF5A 153 residues - Formula weight is not available

1

MET

ALA

ASP

LEU

ASP

PHE

GLU

THR

GLY

2

ASP

ALA

GLY

ALA

SER

ALA

THR

PHE

PRO

MET

3

GLN

CYS

SER

ALA

LEU

ARG

LYS

ASN

GLY

PHE

4

VAL

LEU

LYS

GLY

ARG

PRO

CYS

LYS

ILE

5

VAL

GLU

MET

SER

THR

SER

LYS

THR

GLY

LYS

6

HIS

GLY

HIS

ALA

LYS

VAL

HIS

LEU

VAL

GLY

7

ILE

ASP

ILE

PHE

THR

GLY

LYS

TYR

GLU

8

ASP

ILE

CYS

PRO

SER

THR

HIS

ASN

MET

ASP

9

VAL

PRO

ASN

ILE

LYS

ARG

ASN

ASP

PHE

GLN

10

LEU

ILE

GLY

ILE

GLN

ASP

GLY

TYR

LEU

SER

11

LEU

GLN

ASP

SER

GLY

GLU

VAL

ARG

GLU

12

ASP

LEU

ARG

LEU

PRO

GLU

GLY

ASP

LEU

GLY

13

LYS

GLU

ILE

GLU

GLN

LYS

TYR

ASP

CYS

GLY

14

GLU

ILE

LEU

ILE

THR

VAL

LEU

SER

ALA

15

MET

THR

GLU

ALA

VAL

ALA

ILE

LYS

16

ALA

MET

ALA

Samples:

sample_2: eIF5A, [U-99% 13C; U-99% 15N], 1.0-1.2 mM; sodium phosphate 100 mM

sample_1: eIF5A, [U-99% 15N], 1.0-1.2 mM; sodium phosphate 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.7; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1

Software:

NMRView v5.0.4, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 800 MHz
Varian INOVA 600 MHz

PDB	3CPF
DBJ	BAB27532 BAE29039 BAF85304
EMBL	CAE12193 CAE12194
GB	AAA58453 AAA86989 AAB29229 AAD14095 AAH00751
REF	NP_001003658 NP_001028853 NP_001088861 NP_001093162 NP_001137232
SP	P10160 P63241 P63242 Q3T1J1 Q6EWQ7
TPG	DAA18838
AlphaFold	P63241 Q6EWQ7 Q3T1J1 P63242 P10160