BMRB Entry 16317

Title:
Solution structure of human interleukin-33
Deposition date:
2009-05-26
Original release date:
2009-07-23
Authors:
Lingel, Andreas; Fairbrother, Wayne
Citation:

Citation: Lingel, Andreas; Fairbrother, Wayne. "NMR assignments of the human cytokine interleukin-33"  Biomol. NMR Assignments 3, 223-225 (2009).
PubMed: 19888696

Assembly members:

Assembly members:
human interleukin-33, polymer, 161 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET101

Data sets:
Data typeCount
13C chemical shifts548
15N chemical shifts151
1H chemical shifts1046

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human interleukin-331

Entities:

Entity 1, human interleukin-33 161 residues - Formula weight is not available

1   GLYSERSERILETHRGLYILESERPROILE
2   THRGLUTYRLEUALASERLEUSERTHRTYR
3   ASNASPGLNSERILETHRPHEALALEUGLU
4   ASPGLUSERTYRGLUILETYRVALGLUASP
5   LEULYSLYSASPGLULYSLYSASPLYSVAL
6   LEULEUSERTYRTYRGLUSERGLNHISPRO
7   SERASNGLUSERGLYASPGLYVALASPGLY
8   LYSMETLEUMETVALTHRLEUSERPROTHR
9   LYSASPPHETRPLEUHISALAASNASNLYS
10   GLUHISSERVALGLULEUHISLYSCYSGLU
11   LYSPROLEUPROASPGLNALAPHEPHEVAL
12   LEUHISASNMETHISSERASNCYSVALSER
13   PHEGLUCYSLYSTHRASPPROGLYVALPHE
14   ILEGLYVALLYSASPASNHISLEUALALEU
15   ILELYSVALASPSERSERGLUASNLEUCYS
16   THRGLUASNILELEUPHELYSLEUSERGLU
17   THR

Samples:

15N_sample: Interleukin-33, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 5 mM; H2O 95%; D2O 5%

unlabeled_sample: Interleukin-33 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol, [U-100% 2H], 5 mM; D2O 100%

15N_13C_sample: Interleukin-33, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 5 mM; H2O 95%; D2O 5%

15N_13C_sample_D2O: Interleukin-33, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol, [U-100% 2H], 5 mM; D2O 100%

Condition_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY15N_sampleisotropicCondition_1
3D 1H-13C NOESY15N_13C_sample_D2OisotropicCondition_1
3D HNHA15N_sampleisotropicCondition_1
2D 1H-1H NOESYunlabeled_sampleisotropicCondition_1
3D HNCA15N_13C_sampleisotropicCondition_1
3D HNCACB15N_13C_sampleisotropicCondition_1
3D CBCA(CO)NH15N_13C_sampleisotropicCondition_1
3D C(CO)NH15N_13C_sampleisotropicCondition_1
3D H(CCO)NH15N_13C_sampleisotropicCondition_1
3D HCCH-TOCSY15N_13C_sample_D2OisotropicCondition_1
2D 1H-15N HSQC15N_sampleisotropicCondition_1
2D 1H-13C HSQC15N_13C_sampleisotropicCondition_1

Software:

TOPSPIN, Bruker - collection

CCPN, CCPN - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

ARIA v2.2, Linge, O'Donoghue and Nilges - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

Molmol, Koradi, Billeter and Wuthrich - Display

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz
  • Bruker DRX 900 MHz

Related Database Links:

PDB
DBJ BAA75892 BAG36208 BAG58697 BAJ20898
EMBL CAG28547
GB AAH47085 AAX86998 ADR77828 AIC52722 EAW58748
REF NP_001186570 NP_001300973 NP_001300974 NP_001300975 NP_001300976
SP O95760
AlphaFold O95760

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks