BMRB Entry 16610

Title:
Solution structure of the N-terminal domain (residues 1-111) of Brugia malayi asparaginyl-tRNA synthetase
Deposition date:
2009-11-16
Original release date:
2012-08-03
Authors:
Volkman, B.; Peterson, F.; Kron, M.
Citation:

Citation: Kron, Michael; Volkman, Brian; Peterson, Francis. "TBD"  To be published ., .-..

Assembly members:

Assembly members:
AsnRS, polymer, 113 residues, 12667.689 Da.

Natural source:

Natural source:   Common Name: B. malayi   Taxonomy ID: 6279   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Brugia malayi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-8HT

Data sets:
Data typeCount
13C chemical shifts505
15N chemical shifts124
1H chemical shifts846

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AsnRS1

Entities:

Entity 1, AsnRS 113 residues - 12667.689 Da.

1   GLYSERMETTHRVALTYRILECYSPROGLU
2   THRGLYASPASPGLYASNASPGLYSERGLU
3   LEULYSPROLEUARGTHRLEUTYRGLNALA
4   METILEILETHRLYSSERSERLYSGLYASP
5   PHELEUILEARGTHRLYSLYSASPGLYLYS
6   GLNVALTRPGLUALAALASERLYSTHRALA
7   LEULYSLYSSERTRPLYSARGTYRGLUGLN
8   GLUMETLEULYSASNGLULYSVALALAALA
9   LYSMETLEUGLULYSASPALATHRGLUVAL
10   GLYVALLYSALAALALEUGLUGLUALALYS
11   LYSVALGLNILEGLULEUASPTHRSERLEU
12   SERTYRILE

Samples:

sample_1: AsnRS, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 53 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis

GARANT v2.2, Bartels, Guntert, Billeter and Wuthrich - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance III 500 MHz

Related Database Links:

PDB
EMBL CDQ01803 CDQ01804 CDQ05032 CDQ06500
GB AAA27849 AAA27852
REF XP_001898693 XP_002372115
SP P10723
AlphaFold P10723

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks