BMRB Entry 16664

Title:
Backbone 1H, 13C and 15N resonance assignments for lysozyme from bacteriophage lambda
Deposition date:
2010-01-04
Original release date:
2010-05-05
Authors:
Di Paolo, Alexandre; Matagne, Andre; Redfield, Christina
Citation:

Citation: Di Paolo, Alexandre; Duval, Valerie; Matagne, Andre; Redfield, Christina. "Backbone 1H, 13C, and 15N resonance assignments for lysozyme from bacteriophage lambda."  Biomol. NMR Assignments 4, 111-114 (2010).
PubMed: 20300891

Assembly members:

Assembly members:
lambda_lysozyme, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacteriophage lambda   Taxonomy ID: 10710   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambda-like viruses Enterobacteria phage lambda

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts151
1H chemical shifts315

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lambda lysozyme1

Entities:

Entity 1, lambda lysozyme 158 residues - Formula weight is not available

1   METVALGLUILEASNASNGLNARGLYSALA
2   PHELEUASPMETLEUALATRPSERGLUGLY
3   THRASPASNGLYARGGLNLYSTHRARGASN
4   HISGLYTYRASPVALILEVALGLYGLYGLU
5   LEUPHETHRASPTYRSERASPHISPROARG
6   LYSLEUVALTHRLEUASNPROLYSLEULYS
7   SERTHRGLYALAGLYARGTYRGLNLEULEU
8   SERARGTRPTRPASPALATYRARGLYSGLN
9   LEUGLYLEULYSASPPHESERPROLYSSER
10   GLNASPALAVALALALEUGLNGLNILELYS
11   GLUARGGLYALALEUPROMETILEASPARG
12   GLYASPILEARGGLNALAILEASPARGCYS
13   SERASNILETRPALASERLEUPROGLYALA
14   GLYTYRGLYGLNPHEGLUHISLYSALAASP
15   SERLEUILEALALYSPHELYSGLUALAGLY
16   GLYTHRVALARGGLUILEASPVAL

Samples:

sample_1: lambda lysozyme, [U-98% 15N], 1 mM

sample_2: lambda lysozyme, [U-98% 13C; U-98% 15N], 1 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.45; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D (H)C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

FELIX v2.3, Accelrys Software Inc. - processing

NMR spectrometers:

  • home-built home-built 750 MHz
  • home-built home-built 600 MHz

Related Database Links:

BMRB 19127
PDB
DBJ BAI26565
EMBL CAP75477 CAQ31245 CAR03799 CBG33651 CCK47619
GB AAA96598 AAF30387 AAF31145 AAK28884 AAQ12247
PRF 711077A
REF NP_037696 NP_037753 NP_040645 NP_112069 NP_958236
SP P03706
AlphaFold P03706

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks