BMRB Entry 16883

Title:
the N-terminal domain of human H-REV107
Deposition date:
2010-04-20
Original release date:
2010-06-15
Authors:
Ren, Xiaobai; Xia, Bin
Citation:

Citation: Ren, Xiaobai; Lin, Jian; Jin, Changwen; Xia, Bin. "1H, 13C and 15N resonance assignments of human H-REV107 N-terminal domain."  Biomol. NMR Assignments 4, 175-178 (2010).
PubMed: 20526701

Assembly members:

Assembly members:
H-REV107N, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts116
1H chemical shifts800

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H-REV107N1

Entities:

Entity 1, H-REV107N 125 residues - Formula weight is not available

1   METARGALAPROILEPROGLUPROLYSPRO
2   GLYASPLEUILEGLUILEPHEARGPROPHE
3   TYRARGHISTRPALAILETYRVALGLYASP
4   GLYTYRVALVALHISLEUALAPROPROSER
5   GLUVALALAGLYALAGLYALAALASERVAL
6   METSERALALEUTHRASPLYSALAILEVAL
7   LYSLYSGLULEULEUTYRASPVALALAGLY
8   SERASPLYSTYRGLNVALASNASNLYSHIS
9   ASPASPLYSTYRSERPROLEUPROCYSSER
10   LYSILEILEGLNARGALAGLUGLULEUVAL
11   GLYGLNGLUVALLEUTYRLYSLEUTHRSER
12   GLUASNCYSGLUHISPHEVALASNGLULEU
13   ARGTYRGLYVALALA

Samples:

sample_1: H-REV107N, [U-13C; U-15N], 1 mM; sodium phosphate 30 mM; sodium chloride 30 mM; DTT 10 mM; H2O 90%; D2O 10%; sodium azide 0.05 w/v; DSS 0.02 w/v

sample_conditions_1: ionic strength: 60 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

NMRPipe v2.1, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5, Johnson, One Moon Scientific - data analysis

xwinnmr v3.5, Bruker Biospin - collection

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB08108 BAG35901 BAH08749 BAI46481
EMBL CAA63423 CAH92805
GB AAH01387 AAI03808 AAI03809 AAL26892 ABM83494
REF NP_001121675 NP_001128980 NP_001244356 NP_009000 XP_002833696
SP P53816 Q5R611
AlphaFold P53816 Q5R611

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks