BMRB Entry 16949

Title:
PDZ3 of ZO-1
Deposition date:
2010-05-24
Original release date:
2012-08-02
Authors:
Wang, Xingsheng; Tash, Brian; Flanagan, John; Tian, Fang
Citation:

Citation: Wang, Xingsheng; Tash, Brian; M. Flanagan, John; Tian, Fang. "Protein Backbone Resonance Assignment Assisted with Predicted Local Structures"  .

Assembly members:

Assembly members:
PDZ3_of_ZO-1, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts98
1H chemical shifts98
residual dipolar couplings170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ3_of_ZO-11

Entities:

Entity 1, PDZ3_of_ZO-1 113 residues - Formula weight is not available

1   GLYHISMETPROASNSERTHRHISGLUASP
2   GLYILELEUARGPROSERMETLYSLEUVAL
3   LYSPHEARGLYSGLYASPSERVALGLYLEU
4   ARGLEUALAGLYGLYASNASPVALGLYILE
5   PHEVALALAGLYVALLEUGLUASPSERPRO
6   ALAALALYSGLUGLYLEUGLUGLUGLYASP
7   GLNILELEUARGVALASNASNVALASPPHE
8   THRASNILEILEARGGLUGLUALAVALLEU
9   PHELEULEUASPLEUPROLYSGLYGLUGLU
10   VALTHRILELEUALAGLNLYSLYSLYSASP
11   VALTYRARGARGILEVALGLUSERASPVAL
12   GLYASPSER

Samples:

sample_1: PDZ3 of ZO-1, [U-100% 13C; U-100% 15N], 1 mM; PDZ3 of ZO-1, [U-100% 15N], 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA03274
EMBL CAC41969 CAC41992 CAH18091
GB ADB79761 KFO12263 KFP69448 KFP80555 KFW05584
REF XP_002825269 XP_005196258 XP_005196259 XP_005196260 XP_005196261
TPG DAA17567

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks