BMRB Entry 16965

Title:
Backbone NMR assignment of the globular domain of HET-s(1-227) prion protein.
Deposition date:
2010-05-30
Original release date:
2010-06-30
Authors:
Schuetz, Anne; Greenwald, Jason; Riek, Roland; Bockmann, Anja; Meier, Beat
Citation:

Citation: Schuetz, Anne; Wasmer, Christian; Habenstein, Birgit; Verel, Rene; Greenwald, Jason; Riek, Roland; Bockmann, Anja; Meier, Beat. "Protocols for the sequential solid-state NMR spectroscopic assignment of a uniformly labeled 25 kDa protein: HET-s(1-227)."  Chembiochem 11, 1543-1551 (2010).
PubMed: 20572250

Assembly members:

Assembly members:
HET-s, polymer, 229 residues, 25470 Da.

Natural source:

Natural source:   Common Name: ascomycetes   Taxonomy ID: 5145   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Podospora anserina

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HET-s(1-227)1

Entities:

Entity 1, HET-s(1-227) 229 residues - 25470 Da.

The first two residues represent non-native remainders of a cleaved HIS-tag. This is the globular domain of a prion protein.

1   GLYSERMETSERGLUPROPHEGLYILEVAL
2   ALAGLYALALEUASNVALALAGLYLEUPHE
3   ASNASNCYSVALASPCYSPHEGLUTYRVAL
4   GLNLEUGLYARGPROPHEGLYARGASPTYR
5   GLUARGCYSGLNLEUARGLEUASPILEALA
6   LYSALAARGLEUSERARGTRPGLYGLUALA
7   VALLYSILEASNASPASPPROARGPHEHIS
8   SERSERALAPROTHRASPLYSSERVALGLN
9   LEUALALYSSERILEVALGLUGLUILELEU
10   LEULEUPHEGLUSERALAGLNLYSTHRSER
11   LYSARGTYRGLULEUVALALAASPGLNGLN
12   ASPLEUVALVALPHEGLUASPLYSASPMET
13   LYSPROILEGLYARGALALEUHISARGARG
14   LEUASNASPLEUVALSERARGARGGLNLYS
15   GLNTHRSERLEUALALYSLYSTHRALATRP
16   ALALEUTYRASPGLYLYSSERLEUGLULYS
17   ILEVALASPGLNVALALAARGPHEVALASP
18   GLULEUGLULYSALAPHEPROILEGLUALA
19   VALCYSHISLYSLEUALAGLUILEGLUILE
20   GLUGLUVALGLUASPGLUALASERLEUTHR
21   ILELEULYSASPALAALAGLYGLYILEASP
22   ALAALAMETSERASPALAALAALAGLNLYS
23   ILEASPALAILEVALGLYARGASNSER

Samples:

sample_1: HET-s, [U-100% 13C; U-100% 15N; U-99% 2H], 1 mM; DTT 2 mM; potassium phosphate 50 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 7; pressure: 1 atm; temperature: 297 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16964
PDB
GB AAB19707 AAB94631
PRF 1718317A
SP Q03689
AlphaFold Q03689