BMRB Entry 17232

Title:
Solution structure of the PlyG catalytic domain
Deposition date:
2010-10-01
Original release date:
2012-07-25
Authors:
Volkman, B.; Dias, J.; Peterson, F.
Citation:

Citation: Dias, J.; Peterson, F.; Volkman, B.. "TBD"  To be published ., .-..

Assembly members:

Assembly members:
PlyG, polymer, 165 residues, 18555.006 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Bacillus phage Gamma   Taxonomy ID: 347962   Superkingdom: viruses   Kingdom: not available   Genus/species: not available Bacillus phage Gamma

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAD18

Data sets:
Data typeCount
13C chemical shifts530
15N chemical shifts183
1H chemical shifts1134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PlyG1
2ZINC2

Entities:

Entity 1, PlyG 165 residues - 18555.006 Da.

1   METGLUILEGLNLYSLYSLEUVALASPPRO
2   SERLYSTYRGLYTHRLYSCYSPROTYRTHR
3   METLYSPROLYSTYRILETHRVALHISASN
4   THRTYRASNASPALAPROALAGLUASNGLU
5   VALSERTYRMETILESERASNASNASNGLU
6   VALSERPHEHISILEALAVALASPASPLYS
7   LYSALAILEGLNGLYILEPROLEUGLUARG
8   ASNALATRPALACYSGLYASPGLYASNGLY
9   SERGLYASNARGGLNSERILESERVALGLU
10   ILECYSTYRSERLYSSERGLYGLYASPARG
11   TYRTYRLYSALAGLUASPASNALAVALASP
12   VALVALARGGLNLEUMETSERMETTYRASN
13   ILEPROILEGLUASNVALARGTHRHISGLN
14   SERTRPSERGLYLYSTYRCYSPROHISARG
15   METLEUALAGLUGLYARGTRPGLYALAPHE
16   ILEGLNLYSVALLYSASNGLYASNVALALA
17   THRTHRSERPROTHR

Entity 2, ZINC - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PlyG, [U-100% 13C; U-100% 15N], 1 mM; Bis-Tris, [U-99% 2H], 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 13 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAM97149 ABA42708 ABA46392 ABA46449 ABA46501
REF WP_001982889 YP_338149 YP_338200 YP_459981 YP_512326

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks