BMRB Entry 17362

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17362

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Title:
Backbone assignments for E2-25K
Deposition date:
2010-12-14
Original release date:
2010-12-16
Authors:
Wilson, Randall; Edmondson, Stephen; Twigg, Pamela
Citation:

Citation: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Helms, Kimberli; Twigg, Pamela. "The E2-25K ubiquitin-associated (UBA) domain aids in polyubiquitin chain synthesis and linkage specificity."  Biochem. Biophys. Res. Commun. 405, 662-666 (2011).
PubMed: 21281599

Assembly members:

Assembly members:
E2-25K, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2-25K: AMADIGSMANIAVQRIKREF KEVLKSEETSKNQIKVDLVD ENFTELRGEIAGPPDTPYEG GRYQLEIKIPETYPFNPPKV RFITKIWHPNISSVTGAICL DILKDQWAAAMTLRTVLLSL QALLAAAEPDDPQDAVVANQ YKQNPEMFKQTARLWAHVYA GAPVSSPEYTKKIENLCAMG FDRNAVIVALSSKSWDVETA TELLLSN

Data sets:
Data typeCount
13C chemical shifts558
15N chemical shifts171
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E2-25K monomer1

Entities:

Entity 1, E2-25K monomer 207 residues - Formula weight is not available

Residues 1-7 represent the remainder of a non-native affinity tag

1   ALAMETALAASPILEGLYSERMETALAASN
2   ILEALAVALGLNARGILELYSARGGLUPHE
3   LYSGLUVALLEULYSSERGLUGLUTHRSER
4   LYSASNGLNILELYSVALASPLEUVALASP
5   GLUASNPHETHRGLULEUARGGLYGLUILE
6   ALAGLYPROPROASPTHRPROTYRGLUGLY
7   GLYARGTYRGLNLEUGLUILELYSILEPRO
8   GLUTHRTYRPROPHEASNPROPROLYSVAL
9   ARGPHEILETHRLYSILETRPHISPROASN
10   ILESERSERVALTHRGLYALAILECYSLEU
11   ASPILELEULYSASPGLNTRPALAALAALA
12   METTHRLEUARGTHRVALLEULEUSERLEU
13   GLNALALEULEUALAALAALAGLUPROASP
14   ASPPROGLNASPALAVALVALALAASNGLN
15   TYRLYSGLNASNPROGLUMETPHELYSGLN
16   THRALAARGLEUTRPALAHISVALTYRALA
17   GLYALAPROVALSERSERPROGLUTYRTHR
18   LYSLYSILEGLUASNLEUCYSALAMETGLY
19   PHEASPARGASNALAVALILEVALALALEU
20   SERSERLYSSERTRPASPVALGLUTHRALA
21   THRGLULEULEULEUSERASN

Samples:

sample_1: E2-25K, [U-100% 13C; U-100% 15N; U-80% 2H], 0.35 mM; DSS 0.12 mM; sodium azide 0.9 % v/v; sodium phosphate 50 mM

sample_2: E2-25K, [U-13C; U-15N], 0.50 mM; DSS 0.12 mM; sodium azide 0.9 % v/v; sodium phosphate 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA24927 BAA78555 BAB24523 BAC29296 BAC33269
EMBL CAG32430
GB AAB19536 AAC50633 AAH02013 AAH22804 AAH41728
REF NP_001005662 NP_001008611 NP_001026711 NP_001072139 NP_001080290
SP P61085 P61086 P61087
TPG DAA28715
AlphaFold P61086 P61085 P61087

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks