BMRB Entry 17718

Title:
Hydrogen Exchange in Unligated and Ligated Straphlococcal Nuclease
Deposition date:
2011-06-17
Original release date:
2012-01-24
Authors:
Markley, John; Loh, Stewart; Prehoda, Kenneth; Wang, Jinfeng
Citation:

Citation: Markley, John; Loh, Stewart; Prehoda, Kenneth; Wang, Jinfeng. "Hydrogen exchange in unligated and ligated staphylococcal nuclease"  Biochemistry 32, 11022-11028 (1993).
PubMed: 8218167

Assembly members:

Assembly members:
Staphylococcal_nuclease, polymer, 149 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
THYMIDINE-3',5'-DIPHOSPHATE, non-polymer, 402.188 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pTSN2cc

Data sets:
Data typeCount
H exchange rates115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Staphylococcal_nuclease1
2Calcium ion2
3pdTp3

Entities:

Entity 1, Staphylococcal_nuclease 149 residues - Formula weight is not available

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUTHRLYSHISPROLYSLYSGLY
6   VALGLULYSTYRGLYPROGLUALASERALA
7   PHETHRLYSLYSMETVALGLUASNALALYS
8   LYSILEGLUVALGLUPHEASPLYSGLYGLN
9   ARGTHRASPLYSTYRGLYARGGLYLEUALA
10   TYRILETYRALAASPGLYLYSMETVALASN
11   GLUALALEUVALARGGLNGLYLEUALALYS
12   VALALATYRVALTYRLYSPROASNASNTHR
13   HISGLUGLNLEULEUARGLYSSERGLUALA
14   GLNALALYSLYSGLULYSLEUASNILETRP
15   SERGLUASPASNALAASPSERGLYGLN

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Entity 3, pdTp - C10 H16 N2 O11 P2 - 402.188 Da.

1   THP

Samples:

sample_1: Staphylococcal_nuclease 3.0 mM; pdTp 9.0 mM; succinate-d4 50 mM; Calcium chloride 18 mM; H2O 100%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D HSMQCsample_1isotropicsample_conditions_1

Software:

xwinnmr vAM500, Bruker - chemical shift assignment

NMR spectrometers:

  • Bruker AM 500 MHz