BMRB Entry 17815

Title:
Backbone 1H, 13C and 15N resonance assignments for IMP1 KH34
Deposition date:
2011-07-29
Original release date:
2012-04-23
Authors:
Harris, Richard; Patel, Vivek; Chao, Jeffery; Girvin, Mark; Singer, Robert
Citation:

Citation: Patel, Vivek; Mitra, Somdeb; Harris, Richard; Buxbaum, Adina; Lionnet, Timothee; Brenowitz, Michael; Girvin, Mark; Levy, Matthew; Almo, Steven; Singer, Robert; Chao, Jeffery. "Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control"  Genes Dev. 26, 43-53 (2012).
PubMed: 22215810

Assembly members:

Assembly members:
IMP1_KH34, polymer, 172 residues, 19196 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22HT

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts127
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IMP1 KH341

Entities:

Entity 1, IMP1 KH34 172 residues - 19196 Da.

Residues M(403) and L(567)-H(574) are cloning artifacts and C-terminal His-tag

1   METGLUGLNGLUMETVALGLNVALPHEILE
2   PROALAGLNALAVALGLYALAILEILEGLY
3   LYSLYSGLYGLNHISILELYSGLNLEUSER
4   ARGPHEALASERALASERILELYSILEALA
5   PROPROGLUTHRPROASPSERLYSVALARG
6   METVALILEILETHRGLYPROPROGLUALA
7   GLNPHELYSALAGLNGLYARGILETYRGLY
8   LYSLEULYSGLUGLUASNPHEPHEGLYPRO
9   LYSGLUGLUVALLYSLEUGLUTHRHISILE
10   ARGVALPROALASERALAALAGLYARGVAL
11   ILEGLYLYSGLYGLYLYSTHRVALASNGLU
12   LEUGLNASNLEUTHRALAALAGLUVALVAL
13   VALPROARGASPGLNTHRPROASPGLUASN
14   ASPGLNVALILEVALLYSILEILEGLYHIS
15   PHETYRALASERGLNMETALAGLNARGLYS
16   ILEARGASPILELEUALAGLNVALLYSGLN
17   GLNHISGLNLYSLEUGLUHISHISHISHIS
18   HISHIS

Samples:

sample_1: IMP1 KH34, [U-13C; U-15N; U-2H], 250 uM; TRIS 25 mM; sodium chloride 300 mM; DSS 1 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 300 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

VNMRJ v2.2D, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis v2.1.2, CCPN - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG52029
GB ABB46294 EHH57936 KFP20603 KFZ57551
REF NP_001153895 XP_002800442 XP_003278799 XP_003414705 XP_003435309

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks