BMRB Entry 17959

Title:
Backbone and side chain 1H, 13C, and 15N assignments of the ubiquitin-like domain of human HOIL-1L
Deposition date:
2011-09-26
Original release date:
2011-12-12
Authors:
Uekusa, Yoshinori; Mimura, Syunsuke; Sasakawa, Hiroaki; Kurimoto, Eiji; Sakata, Eri; Olivier, Serve; Yagi, Hirokazu; Tokunaga, Fumimori; Iwai, Kazuhiro; Kato, Koichi
Citation:

Citation: Uekusa, Yoshinori; Mimura, Syunsuke; Sasakawa, Hiroaki; Kurimoto, Eiji; Sakata, Eri; Olivier, Serve; Yagi, Hirokazu; Tokunaga, Fuminori; Iwai, Kazuhiro; Kato, Koichi. "Backbone and side chain 1H, 13C, and 15N assignments of the ubiquitin-like domain of human HOIL-1L, an essential component of linear ubiquitin chain assembly complex."  Biomol. NMR Assignments 6, 177-180 (2012).
PubMed: 22127525

Assembly members:

Assembly members:
HOIL-1L, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Data sets:
Data typeCount
13C chemical shifts513
15N chemical shifts121
1H chemical shifts804

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human HOIL-1L1

Entities:

Entity 1, human HOIL-1L 129 residues - Formula weight is not available

Residues 1-4 represent an expression vector fragment.

1   GLYSERHISMETGLNARGVALPROLEUSER
2   VALGLNLEULYSPROGLUVALSERPROTHR
3   GLNASPILEARGLEUTRPVALSERVALGLU
4   ASPALAGLNMETHISTHRVALTHRILETRP
5   LEUTHRVALARGPROASPMETTHRVALALA
6   SERLEULYSASPMETVALPHELEUASPTYR
7   GLYPHEPROPROVALLEUGLNGLNTRPVAL
8   ILEGLYGLNARGLEUALAARGASPGLNGLU
9   THRLEUHISSERHISGLYVALARGGLNASN
10   GLYASPSERALATYRLEUTYRLEULEUSER
11   ALAARGASNTHRSERLEUASNPROGLNGLU
12   LEUGLNARGGLUARGGLNLEUARGMETLEU
13   GLNASPLEUGLYPHELYSASPLEUTHR

Samples:

sample_1: acetic acid 10 mM; HOIL-1L, [U-100% 13C; U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 4.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.1.5, CCPN - chemical shift assignment

NMR spectrometers:

  • JEOL ECA 600 MHz

Related Database Links:

BMRB 17828
PDB
DBJ BAC75409 BAG59927 BAG64417
GB AAD00162 AAH15219 AIC59659 EAX10673 EAX10675
REF NP_001253226 NP_006453 NP_112506 XP_001152050 XP_002830204
SP Q9BYM8
AlphaFold Q9BYM8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks