BMRB Entry 18235

Title:
Solution Structure Of The N-Terminal Domain Of The Yeast Protein Dre2
Deposition date:
2012-02-01
Original release date:
2015-07-24
Authors:
Craescu, Constantin; Vernis, Laurence; Pastore, Annalisa
Citation:

Citation: Soler, Nicolas; Craescu, Costantin; Gallay, Jacques; Frapart, Yves-Michel; Mansuy, Daniel; Raynal, Bertrand; Baldacci, Giuseppe; Pastore, Annalisa; Huang, Meng-Er; Vernis, Laurence. "A S-adenosylmethionine methyltransferase-like domain within the essential, Fe-S-containing yeast protein Dre2"  FEBS J. 279, 2108-2119 (2012).
PubMed: 22487307

Assembly members:

Assembly members:
N-dre2, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSKB3

Data sets:
Data typeCount
13C chemical shifts466
15N chemical shifts127
1H chemical shifts873

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-dre21

Entities:

Entity 1, N-dre2 136 residues - Formula weight is not available

The first three residues are a tag. Numbering of the entry starts at the second Met, that is the natural sequence.

1   GLYHISMETMETSERGLNTYRLYSTHRGLY
2   LEULEULEUILEHISPROALAVALTHRTHR
3   THRPROGLULEUVALGLUASNTHRLYSALA
4   GLNALAALASERLYSLYSVALLYSPHEVAL
5   ASPGLNPHELEUILEASNLYSLEUASNASP
6   GLYSERILETHRLEUGLUASNALALYSTYR
7   GLUTHRVALHISTYRLEUTHRPROGLUALA
8   GLNTHRASPILELYSPHEPROLYSLYSLEU
9   ILESERVALLEUALAASPSERLEULYSPRO
10   ASNGLYSERLEUILEGLYLEUSERASPILE
11   TYRLYSVALASPALALEUILEASNGLYPHE
12   GLUILEILEASNGLUPROASPTYRCYSTRP
13   ILELYSMETASPSERSERLYSLEUASNGLN
14   THRVALSERILEPROLEU

Samples:

sample_1: potassium phosphate 50 mM; DTT 1 mM; sodium chloride 50 mM; N-dre2 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.100 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR, Accelrys Software Inc. - structure solution

NMR spectrometers:

  • Varian Unity 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ GAA24792
EMBL CAA82150 CAY81149
GB AHY76305 AJP40099 AJS30297 AJS30598 AJS30896
REF NP_012997
SP A7A031 B3LRE5 C7GX69 C8ZCN3 P36152
TPG DAA09222
AlphaFold C8ZCN3 A7A031 B3LRE5 C7GX69 P36152

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks