BMRB Entry 18855

Title:
Backbone assignments of 8M urea denatured YibK from Haemophilus influenzae (HI0766)
Deposition date:
2012-11-24
Original release date:
2014-02-14
Authors:
Hsieh, Shu-Ju Micky; Hsu, Shang-Te Danny
Citation:

Citation: Hsieh, Shu-Ju Micky; Mallam, Anna; Jackson, Sophie; Hsu, Shang-Te Danny. "Backbone 1H, 13C and 15N assignments of YibK and avariant containing a unique cysteine residue at C-terminus in 8 M urea-denatured states"  Biomol. NMR Assignments 8, 439-442 (2014).
PubMed: 23853076

Assembly members:

Assembly members:
YibK, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet 17b

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YibK1

Entities:

Entity 1, YibK 160 residues - Formula weight is not available

1   METLEUASPILEVALLEUTYRGLUPROGLU
2   ILEPROGLNASNTHRGLYASNILEILEARG
3   LEUCYSALAASNTHRGLYPHEARGLEUHIS
4   LEUILEGLUPROLEUGLYPHETHRTRPASP
5   ASPLYSARGLEUARGARGSERGLYLEUASP
6   TYRHISGLUPHEALAGLUILELYSARGHIS
7   LYSTHRPHEGLUALAPHELEUGLUSERGLU
8   LYSPROLYSARGLEUPHEALALEUTHRTHR
9   LYSGLYCYSPROALAHISSERGLNVALLYS
10   PHELYSLEUGLYASPTYRLEUMETPHEGLY
11   PROGLUTHRARGGLYILEPROMETSERILE
12   LEUASNGLUMETPROMETGLUGLNLYSILE
13   ARGILEPROMETTHRALAASNSERARGSER
14   METASNLEUSERASNSERVALALAVALTHR
15   VALTYRGLUALATRPARGGLNLEUGLYTYR
16   LYSGLYALAVALASNLEUPROGLUVALLYS

Samples:

YibK_in_denatured_state: YibK, [U-13C; U-15N], 350 uM; D2O 10%; TRIS 50 mM; potassium chloride 200 mM; glycerol 10%; urea 7.2 M; H2O 90%

SEC-urea: pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCYibK_in_denatured_stateisotropicSEC-urea
3D CBCA(CO)NHYibK_in_denatured_stateisotropicSEC-urea
3D HNCOYibK_in_denatured_stateisotropicSEC-urea
3D HNCACBYibK_in_denatured_stateisotropicSEC-urea
3D HNCAYibK_in_denatured_stateisotropicSEC-urea
3D C(CO)NHYibK_in_denatured_stateisotropicSEC-urea

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks