BMRB Entry 18889

Title:
CD3e cyt
Deposition date:
2012-12-11
Original release date:
2013-10-15
Authors:
Mayzel, Maxim
Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains"  PLoS One 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD3e, polymer, 57 residues, 6390.3331 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Wheat germ - cell freeE. coli - cell free   Vector: pEXP5-NT

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts304
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD3e1

Entities:

Entity 1, CD3e 57 residues - 6390.3331 Da.

1   SERLEULYSASNARGLYSALALYSALALYS
2   PROVALTHRARGGLYALAGLYALAGLYGLY
3   ARGGLNARGGLYGLNASNLYSGLUARGPRO
4   PROPROVALPROASNPROASPTYRGLUPRO
5   ILEARGLYSGLYGLNARGASPLEUTYRSER
6   GLYLEUASNGLNARGARGILE

Samples:

Native: CD3e, [U-100% 13C; U-100% 15N], 0.1 mM; DTT 2.0 mM; EDTA 1.0 mM; potassium phospate 20.0 mM; azide 0.02%; Complete EDTA-free 1.0 x

Denatured: CD3e, [U-100% 13C; U-100% 15N], 0.1 mM; DTT 2.0 mM; EDTA 1.0 mM; potassium phospate 20.0 mM; azide 0.02%; Complete EDTA-free 1 x; Urea 6 M

1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCANativeisotropic1
3D HNCACBNativeisotropic1
3D HN(CA)CONativeisotropic1
3D HNCONativeisotropic1
3D HN(CO)CANativeisotropic1
3D HN(CO)CACBNativeisotropic1
2D 1H-15N HSQC/HMQCNativeisotropic1
2D 1H-15N HSQC/HMQCDenaturedisotropic1
3D HNCADenaturedisotropic1
3D HNCACBDenaturedisotropic1
3D HN(CA)CODenaturedisotropic1
3D HNCODenaturedisotropic1
3D HN(CO)CADenaturedisotropic1
3D HN(CO)CACBDenaturedisotropic1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - refinement

MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing

TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

  • Varian UnityPlus 600 MHz

Related Database Links:

UNP P07766
DBJ BAB71849 BAB71850 BAF85301 BAJ16130 BAJ16131
EMBL CAA27516
GB AAA52295 AAH49847 AAV38740 AAX42814 AAX42815
REF NP_000724 NP_001270544 XP_001097204 XP_002822594 XP_003253275
SP P07766 Q95LI5
AlphaFold A8K997 P07766 Q95LI5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks