BMRB Entry 18908

Title:
Human programmed cell death 1 receptor
Deposition date:
2012-12-18
Original release date:
2013-02-21
Authors:
Veverka, Vaclav; Cheng, Xiaoxiao; Waters, Lorna; Muskett, Frederick; Morgan, Sara; Lesley, Alastair; Griffiths, Merryn; Stubberfield, Colin; Griffin, Robert; Henry, Alistair; Robinson, Martyn; Jansson, Andreas; Ladbury, John; Ikemizu, Shinji; Davis, Simon; Carr, Mark
Citation:

Citation: Cheng, Xiaoxiao; Veverka, Vaclav; Radhakrishnan, Anand; Waters, Lorna; Muskett, Frederick; Morgan, Sara; Huo, Jiandong; Yu, Chao; Evans, Edward; Leslie, Alasdair; Griffiths, Meryn; Stubberfield, Colin; Griffin, Robert; Henry, Alistair; Jansson, Andreas; Ladbury, John; Ikemizu, Shinji; Carr, Mark; Davis, Simon. "Structure and interactions of the human programmed cell death 1 receptor."  J. Biol. Chem. 288, 11771-11785 (2013).
PubMed: 23417675

Assembly members:

Assembly members:
Human programmed cell death 1 receptor, polymer, 118 residues, 13245.914 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts119
1H chemical shifts774

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human programmed cell death 1 receptor1

Entities:

Entity 1, Human programmed cell death 1 receptor 118 residues - 13245.914 Da.

1   METPROPROTHRPHESERPROALALEULEU
2   VALVALTHRGLUGLYASPASNALATHRPHE
3   THRCYSSERPHESERASNTHRSERGLUSER
4   PHEVALLEUASNTRPTYRARGMETSERPRO
5   SERASNGLNTHRASPLYSLEUALAALAPHE
6   PROGLUASPARGSERGLNPROGLYGLNASP
7   SERARGPHEARGVALTHRGLNLEUPROASN
8   GLYARGASPPHEHISMETSERVALVALARG
9   ALAARGARGASNASPSERGLYTHRTYRLEU
10   CYSGLYALAILESERLEUALAPROLYSALA
11   GLNILELYSGLUSERLEUARGALAGLULEU
12   ARGVALTHRGLUARGARGALAGLU

Samples:

sample_N: HPCD1R, [U-15N], 0.5 mM; potassium phosphate 25 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

sample_CN: HPCD1R, [U-13C; U-15N], 0.5 mM; potassium phosphate 25 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

sample_AR: HPCD1R 0.5 mM; potassium phosphate 25 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 0.125 M; pH: 6.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_Nisotropicsample_conditions_1
2D 1H-13C HSQCsample_CNisotropicsample_conditions_1
2D 1H-1H TOCSYsample_ARisotropicsample_conditions_1
2D 1H-1H NOESYsample_ARisotropicsample_conditions_1
3D HNCACBsample_CNisotropicsample_conditions_1
3D CBCA(CO)NHsample_CNisotropicsample_conditions_1
3D HNCOsample_CNisotropicsample_conditions_1
3D HCCH-TOCSYsample_CNisotropicsample_conditions_1
3D 1H-15N TOCSYsample_Nisotropicsample_conditions_1
3D 1H-13C NOESYsample_CNisotropicsample_conditions_1
3D 1H-15N NOESYsample_Nisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAG36577 BAJ21104
GB AAC41700 AAC51773 AAH74740 AAN64003 AAO25116
REF NP_005009 XP_003776178 XP_004033550 XP_006712636 XP_006725049
SP Q15116
AlphaFold Q15116

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks