BMRB Entry 18918

Title:
Chemical shifts assignment for the unfolded state of the N-terminal domain of ribosomal protein L9 (NTL9) V3AI4A double mutant in 8.3 M urea
Deposition date:
2012-12-22
Original release date:
2013-05-30
Authors:
Meng, Wenli; Raleigh, Daniel
Citation:

Citation: Meng, Wenli; Luan, Bowu; Lyle, Nicholas; Pappu, Rohit; Raleigh, Daniel. "The denatured state ensemble contains significant local and long-range structure under native conditions: analysis of the N-terminal domain of ribosomal protein L9."  Biochemistry 52, 2662-2671 (2013).
PubMed: 23480024

Assembly members:

Assembly members:
NTL9_V3AI4A_double_mutant, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NTL9_V3AI4A_double_mutant: MKAAFLKDVKGKGKKGEIKN VADGYANNFLFKQGLAIEAT PANLKALEAQKQKEQR

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts54
1H chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTL9_V3AI4A1

Entities:

Entity 1, NTL9_V3AI4A 56 residues - Formula weight is not available

1   METLYSALAALAPHELEULYSASPVALLYS
2   GLYLYSGLYLYSLYSGLYGLUILELYSASN
3   VALALAASPGLYTYRALAASNASNPHELEU
4   PHELYSGLNGLYLEUALAILEGLUALATHR
5   PROALAASNLEULYSALALEUGLUALAGLN
6   LYSGLNLYSGLUGLNARG

Samples:

sample_1: NTL9 V3AI4A double mutant, [U-99% 13C; U-99% 15N], 0.5 mM; urea 8.3 M

sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17675

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks