BMRB Entry 19049

Title:
Solution structure of chicken Engrailed 2 homeodomain
Deposition date:
2013-02-21
Original release date:
2013-08-14
Authors:
Carlier, Ludovic; Balayssac, Stephane; Cantrelle, Francois-Xavier; Khemtemourian, Lucie; Chassaing, Gerard; Joliot, Alain; Lequin, Olivier
Citation:

Citation: Carlier, Ludovic; Balayssac, Stephane; Cantrelle, Francois-Xavier; Khemtemourian, Ludovic; Chassaing, Gerard; Joliot, Alain; Lequin, Olivier. "Investigation of homeodomain membrane translocation properties: insights from the structure determination of engrailed-2 homeodomain in aqueous and membrane-mimetic environments"  Biophys. J. 105, 667-678 (2013).
PubMed: 23931315

Assembly members:

Assembly members:
HOMEOBOX_PROTEIN_ENGRAILED-2, polymer, 67 residues, 7857.0148 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HOMEOBOX_PROTEIN_ENGRAILED-2: GPMASDKRPRTAFTAEQLQR LKAEFQTNRYLTEQRRQSLA QELGLNESQIKIWFQNKRAK IKKATQA

Data sets:
Data typeCount
13C chemical shifts332
15N chemical shifts85
1H chemical shifts410

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HOMEOBOX PROTEIN ENGRAILED-21

Entities:

Entity 1, HOMEOBOX PROTEIN ENGRAILED-2 67 residues - 7857.0148 Da.

1   GLYPROMETALASERASPLYSARGPROARG
2   THRALAPHETHRALAGLUGLNLEUGLNARG
3   LEULYSALAGLUPHEGLNTHRASNARGTYR
4   LEUTHRGLUGLNARGARGGLNSERLEUALA
5   GLNGLULEUGLYLEUASNGLUSERGLNILE
6   LYSILETRPPHEGLNASNLYSARGALALYS
7   ILELYSLYSALATHRGLNALA

Samples:

sample_1: HOMEOBOX PROTEIN ENGRAILED-2 1 mM; D2O 10%; sodium azide 0.02%; sodium succinate 50 mM; TMSP 0.1 mM; H2O 90%

sample_2: HOMEOBOX PROTEIN ENGRAILED-2, [U-15N], 1 mM; D2O 10%; sodium azide 0.02%; sodium succinate 50 mM; TMSP 0.1 mM; H2O 90%

sample_3: HOMEOBOX PROTEIN ENGRAILED-2, [U-13C; U-15N], 1 mM; D2O 10%; sodium azide 0.02%; sodium succinate 50 mM; TMSP 0.1 mM; H2O 90%

sample_conditions_1: ionic strength: 50.000 mM; pH: 4.700; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2solutionsample_conditions_1
3D HNCACBsample_3solutionsample_conditions_1
3D CBCA(CO)NHsample_3solutionsample_conditions_1
3D HBHA(CO)NHsample_3solutionsample_conditions_1
3D HNCAsample_3solutionsample_conditions_1
3D HN(CO)CAsample_3solutionsample_conditions_1
3D HNCOsample_3solutionsample_conditions_1
3D HN(CA)COsample_3solutionsample_conditions_1
3D 15N TOCSY-HSQCsample_2solutionsample_conditions_1
3D HCCH TOCSYsample_3solutionsample_conditions_1
2D (HB)CB(CGCD)HDsample_3solutionsample_conditions_1
2D NOESYsample_1solutionsample_conditions_1
3D 15N NOESY-HSQCsample_2solutionsample_conditions_1
3D 13C NOESY-HMQCsample_3solutionsample_conditions_1
3D HNHAsample_2solutionsample_conditions_1
3D HNHBsample_2solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

DYANA vany, Guntert, Braun and Wuthrich - chemical shift assignment

X-PLOR NIH vany, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

UNP HME2_CHICK
BMRB 17325
PDB
DBJ BAE34186
EMBL CAA44724 CAA48254 CAA48491 CAA68362
GB AAA53438 AAA53504 AAA53527 AAF31303 AAF68670
PRF 1703276B 1703276F
REF NP_001095261 NP_001102684 NP_001254648 NP_001418 NP_034264
SP P09015 P09066 P19622 P52730 Q05917
TPG DAA30339
AlphaFold Q05917 P09015 P09066 P19622 P52730 Q05917

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks