BMRB Entry 19055

Title:
ns5a D2 con1
Deposition date:
2013-02-26
Original release date:
2017-01-30
Authors:
Badillo, Aurelie; Penin, Francois
Citation:

Citation: Badillo, Aurelie; Hanoulle, Xavier; Penin, Francois. "ns5a D2 con1"  Biochemistry ., .-..

Assembly members:

Assembly members:
ns5a_d2_con1, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pt7-7

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts85
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ns5a d2 con11

Entities:

Entity 1, ns5a d2 con1 96 residues - Formula weight is not available

1   ASPSERPROASPALAASPLEUILEGLUALA
2   ASNLEULEUTRPARGGLNGLUMETGLYGLY
3   ASNILETHRARGVALGLUSERGLUASNLYS
4   VALVALILELEUASPSERPHEGLUPROLEU
5   GLNALAGLUGLUASPGLUARGGLUVALSER
6   VALPROALAGLUILELEUARGARGSERARG
7   LYSPHEPROARGALAMETPROILETRPALA
8   ARGPROASPTYRASNPROPROLEULEUGLU
9   SERTRPLYSASPPROASPTYRVALPROPRO
10   VALVALHISGLYLEUGLN

Samples:

sample_1: ns5a d2 con1, [U-100% 13C; U-100% 15N], 0.5 ± 0.1 mM; H2O 95 ± 0.1 %; D2O 5 ± 0.1 %

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks