BMRB Entry 19108

Title:
NMR structure of human restriction factor APOBEC3A
Deposition date:
2013-03-21
Original release date:
2013-05-20
Authors:
Byeon, In-Ja; Byeon, Chang-Hyeock; Ahn, Jinwoo; Gronenborn, Angela
Citation:

Citation: Byeon, In-Ja; Ahn, Jinwoo; Mitra, Mithun; Byeon, Chang-Hyeock; Hercik, Kamil; Hritz, Jozef; Charlton, Lisa; Levin, Judith; Gronenborn, Angela. "NMR structure of human restriction factor APOBEC3A reveals substrate binding and enzyme specificity."  Nat. Commun. 4, 1890-1890 (2013).
PubMed: 23695684

Assembly members:

Assembly members:
entity_1, polymer, 199 residues, 23041.172 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Data sets:
Data typeCount
13C chemical shifts502
15N chemical shifts203
1H chemical shifts1146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human restriction factor APOBEC3A1
2ZINC ION2

Entities:

Entity 1, human restriction factor APOBEC3A 199 residues - 23041.172 Da.

1   METGLUALASERPROALASERGLYPROARG
2   HISLEUMETASPPROHISILEPHETHRSER
3   ASNPHEASNASNGLYILEGLYARGHISLYS
4   THRTYRLEUCYSTYRGLUVALGLUARGLEU
5   ASPASNGLYTHRSERVALLYSMETASPGLN
6   HISARGGLYPHELEUHISASNGLNALALYS
7   ASNLEULEUCYSGLYPHETYRGLYARGHIS
8   ALAGLULEUARGPHELEUASPLEUVALPRO
9   SERLEUGLNLEUASPPROALAGLNILETYR
10   ARGVALTHRTRPPHEILESERTRPSERPRO
11   CYSPHESERTRPGLYCYSALAGLYGLUVAL
12   ARGALAPHELEUGLNGLUASNTHRHISVAL
13   ARGLEUARGILEPHEALAALAARGILETYR
14   ASPTYRASPPROLEUTYRLYSGLUALALEU
15   GLNMETLEUARGASPALAGLYALAGLNVAL
16   SERILEMETTHRTYRASPGLUPHELYSHIS
17   CYSTRPASPTHRPHEVALASPHISGLNGLY
18   CYSPROPHEGLNPROTRPASPGLYLEUASP
19   GLUHISSERGLNALALEUSERGLYARGLEU
20   ARGALAILELEUGLNASNGLNGLYASN

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_2: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_3: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_4: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_5: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_6: APOBEC3A, [U-13C; U-15N], mM; ZINC ION mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: . K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: . K

sample_conditions_3: pH: 6.5; pressure: 1 atm; temperature: . K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D simulaneous 1H-13C and 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D HNCAsample_2isotropicsample_conditions_2
3D HN(CO)CAsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D simultaneous 1H-13C and 1H-15N NOESYsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_3isotropicsample_conditions_3
3D HCCH-TOCSYsample_3isotropicsample_conditions_3
3D simulaneous 1H-13C and 1H-15N NOESYsample_3isotropicsample_conditions_3
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D HN(CO)CACBsample_4isotropicsample_conditions_1
3D simultaneous 1H-13C and 1H-15N NOESYsample_4isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_5isotropicsample_conditions_1
3D simultaneous 1H-13C and 1H-15N NOESYsample_5isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_5isotropicsample_conditions_1
2D 1H-1H NOESYsample_6isotropicsample_conditions_1
2D 1H-1H TOCSYsample_6isotropicsample_conditions_1

Software:

TOPSPIN v2.3 -

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAI46227
EMBL CAG30279 CAK54381 CAK54680
GB AAA03706 AAI26417 ADO85886 ADR82854 AIC53372
REF NP_001180218 NP_663745 XP_001149270 XP_011545340 XP_011545341
SP P31941
AlphaFold P31941

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks