BMRB Entry 19140

Title:
Dynorphin A R6W
Deposition date:
2013-04-04
Original release date:
2013-05-06
Authors:
Bjorneras, Johannes; Graslund, Astrid; Maler, Lena
Citation:

Citation: Bjorneras, Johannes; Graslund, Astrid; Maler, Lena. "Membrane interaction of disease-related dynorphin a variants."  Biochemistry 52, 4157-4167 (2013).
PubMed: 23705820

Assembly members:

Assembly members:
R6W-DynA, polymer, 17 residues, 2177 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R6W-DynA: YGGFLWRIRPKLKWDNQ

Data typeCount
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R6W-DynA1

Entities:

Entity 1, R6W-DynA 17 residues - 2177 Da.

1   TYRGLYGLYPHELEUTRPARGILEARGPRO
2   LYSLEULYSTRPASPASNGLN

Samples:

sample_1: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM

sample_2: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DMPC, [U-2H], 35 mM; DHPC, [U-2H], 115 mM

sample_3: R6W-DynA 1 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DMPG, [U-2H], 7 mM; DMPC, [U-2H], 28 mM; DHPC, [U-2H], 115 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 5.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz