BMRB Entry 19184

Title:
solution structure of the calmodulin-binding domain of plant calcium-ATPase ACA2
Deposition date:
2013-04-21
Original release date:
2014-04-22
Authors:
Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans
Citation:

Citation: Jamshidiha, Mostafa; Ishida, Hiroaki; Gifford, Jessica; Vogel, Hans. "Structural characterization of the interactions between calmodulin and three distinct plant calcium-ATPase pumps"  .

Assembly members:

Assembly members:
ACA2, polymer, 26 residues, 3139.773 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ACA2: VLEKWRNLCGVVKNPKRRFR FTANLS

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACA21

Entities:

Entity 1, ACA2 26 residues - 3139.773 Da.

1   VALLEUGLULYSTRPARGASNLEUCYSGLY
2   VALVALLYSASNPROLYSARGARGPHEARG
3   PHETHRALAASNLEUSER

Samples:

sample_1: D2O, [U-100% 2H], 10%; DSS, [U-100% 2H], 5 mM; DTT, [U-100% 2H], 10 mM; H2O 60%; TFE, [U-100% 2H], 30%

sample_conditions_1: ionic strength: 0 M; pH: 4.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
EMBL CAB38303 CAB80429 CDX72644 CDX76883 CDY22420
GB AAC26997 AAL32562 AAQ89614 ADR82620 AEE86819
REF NP_195479 XP_006283060 XP_006283061 XP_006426128 XP_006466431
SP O81108
AlphaFold O81108