BMRB Entry 19335

Title:
NMR assignments and relaxation of 5P12-RANTES-E66S
Deposition date:
2013-07-03
Original release date:
2014-02-04
Authors:
Wiktor, Maciej; Grzesiek, Stephan
Citation:

Citation: Wiktor, Maciej; Hartley, Oliver; Grzesiek, Stephan. "Characterization of structure, dynamics, and detergent interactions of the anti-HIV chemokine variant 5P12-RANTES"  Biophys. J. 105, 2586-2597 (2013).
PubMed: 24314089

Assembly members:

Assembly members:
5P12-RANTES-E66S, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEV2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts68
1H chemical shifts63
heteronuclear NOE values63
T1 relaxation values63
T2 relaxation values63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15P12-RANTES-E66S1

Entities:

Entity 1, 5P12-RANTES-E66S 69 residues - Formula weight is not available

1   GLNGLYPROPROLEUMETALATHRGLNSER
2   CYSCYSPHEALATYRILEALAARGPROLEU
3   PROARGALAHISILELYSGLUTYRPHETYR
4   THRSERGLYLYSCYSSERASNPROALAVAL
5   VALPHEVALTHRARGLYSASNARGGLNVAL
6   CYSALAASNPROGLULYSLYSTRPVALARG
7   GLUTYRILEASNSERLEUSERMETSER

Samples:

sample_1: 5P12-RANTES-E66S, [U-98% 13C; U-98% 15N], 0.6 mM; potassium phosphate 50 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: pH: 3.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
15N T1sample_1isotropicsample_conditions_1
15N T2sample_1isotropicsample_conditions_1
{1H}-15N NOEsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

PIPP, Garrett - data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 19336
GB AAN76984

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks