BMRB Entry 19367

Title:
Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 non template element TGTCAAA
Deposition date:
2013-07-16
Original release date:
2014-02-10
Authors:
Campagne, Sebastien; Vorholt, Julia; Allain, Frederic
Citation:

Citation: Campagne, Sebastien; Marsh, May; Capitani, Guido; Vorholt, Julia; Allain, Frederic H-T. "Structural basis for -10 promoter element melting by environmentally induced sigma factors."  Nat. Struct. Mol. Biol. 21, 269-276 (2014).
PubMed: 24531660

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, 11024.694 Da.
entity_2, polymer, 7 residues, 11024.694 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26BII

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts103
1H chemical shifts684

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1region 2 of E. coli sigmaE1
2non template strand2

Entities:

Entity 1, region 2 of E. coli sigmaE 96 residues - 11024.694 Da.

1   METSERGLUGLNLEUTHRASPGLNVALLEU
2   VALGLUARGVALGLNLYSGLYASPGLNLYS
3   ALAPHEASNLEULEUVALVALARGTYRGLN
4   HISLYSVALALASERLEUVALSERARGTYR
5   VALPROSERGLYASPVALPROASPVALVAL
6   GLNGLUALAPHEILELYSALATYRARGALA
7   LEUASPSERPHEARGGLYASPSERALAPHE
8   TYRTHRTRPLEUTYRARGILEALAVALASN
9   THRALALYSASNTYRLEUVALALAGLNGLY
10   ARGARGLEUGLULEUVAL

Entity 2, non template strand 7 residues - 11024.694 Da.

1   DTDGDTDCDADADA

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 0.8 mM; entity_2 0.8 mM; H2O 90%; D2O 10%; NaPO4 10 mM; NaCl 50 mM

sample_2: entity_1, [U-100% 13C; U-100% 15N], 0.8 mM; entity_2 0.8 mM; D2O 100%; NaPO4 10 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D NOESY 1H-1H F1F2Fsample_2isotropicsample_conditions_1
2D NOESY 1H-1H F2Fsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 19366
PDB
DBJ BAA10920 BAB36862 BAE76749 BAG78385 BAI26818
EMBL CAD02789 CAP77019 CAQ32948 CAQ88049 CAQ99523
GB AAA57186 AAC45314 AAC75626 AAG57689 AAL21534
PIR AI0829
REF NP_311466 NP_417068 NP_457116 NP_461575 NP_708425
SP D0ZSY9 P0A2F0 P0A2F1 P0AGB6 P0AGB7
AlphaFold P0AGB7 D0ZSY9 P0A2F0 P0A2F1 P0AGB6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks