BMRB Entry 19382

Name: Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'
Published: 2014-05-06

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PDB ID: 2mb0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19382
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'

Deposition date:

2013-07-22

Original release date:

2014-05-06

Authors:

Moursy, Ahmed; Allain, Frederic; Clery, Antoine

Citation:

Citation: Moursy, Ahmed; Allain, Frederic H-T; Clery, Antoine. "Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation." Nucleic Acids Res. ., .-. (2014).
PubMed: 24692659

Assembly members:

Assembly members:
hnRNP_G_RRM, polymer, 95 residues, 10413.906 Da.
RNA_AUCAAA, polymer, 6 residues, 1883.228 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hnRNP_G_RRM: MVEADRPGKLFIGGLNTETN EKALEAVFGKYGRIVEVLLM KDRETNKSRGFAFVTFESPA DAKDAARDMNGKSLDGKAIK VEQATKPSFESGRRG
RNA_AUCAAA: AUCAAA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	293
15N chemical shifts	97
1H chemical shifts	685

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hnRNP G RRM	1
2	RNA (5'-R(APUPCPAPAPA)-3')	2

Entities:

Entity 1, hnRNP G RRM 95 residues - 10413.906 Da.

1

MET

VAL

GLU

ALA

ASP

ARG

PRO

GLY

LYS

LEU

2

PHE

ILE

GLY

LEU

ASN

THR

GLU

THR

ASN

3

GLU

LYS

ALA

LEU

GLU

ALA

VAL

PHE

GLY

LYS

4

TYR

GLY

ARG

ILE

VAL

GLU

VAL

LEU

MET

5

LYS

ASP

ARG

GLU

THR

ASN

LYS

SER

ARG

GLY

6

PHE

ALA

PHE

VAL

THR

PHE

GLU

SER

PRO

ALA

7

ASP

ALA

LYS

ASP

ALA

ARG

ASP

MET

ASN

8

GLY

LYS

SER

LEU

ASP

GLY

LYS

ALA

ILE

LYS

9

VAL

GLU

GLN

ALA

THR

LYS

PRO

SER

PHE

GLU

10

SER

GLY

ARG

GLY

Entity 2, RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 6 residues - 1883.228 Da.

1

A

U

C

A

Samples:

15N_H2O: hnRNP_G_RRM, [U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; H2O 90%; D2O 10%; NaCl 50 mM; NaH2PO4 20 mM

15N_D2O: hnRNP_G_RRM, [U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; D2O 100%; NaCl 50 mM; NaH2PO4 20 mM

13C_H2O: hnRNP_G_RRM, [U-100% 13C; U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; H2O 90%; D2O 10%; NaCl 50 mM; NaH2PO4 20 mM

13C_D2O: hnRNP_G_RRM, [U-100% 13C; U-100% 15N], 1 mM; RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') 1 mM; D2O 100%; NaCl 50 mM; NaH2PO4 20 mM

sample_conditions_1: ionic strength: 70 mM; pH: 5.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	13C_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	13C_H2O	isotropic	sample_conditions_1
2D 1H-1H TOCSY	15N_D2O	isotropic	sample_conditions_1
2D 1H-1H NOESY	15N_D2O	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C_H2O	isotropic	sample_conditions_1
3D HNCA	13C_H2O	isotropic	sample_conditions_1
3D HNCACB	13C_H2O	isotropic	sample_conditions_1
3D HCCH-TOCSY	13C_H2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	13C_H2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	13C_H2O	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N_H2O	isotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 900 MHz

BMRB	19792
PDB	2MB0 2MKS
DBJ	BAC31099 BAE00972 BAE22448 BAE37998 BAF62396
EMBL	CAA80599 CAB51361 CAB51362 CAG31684 CAI46148
GB	AAH03710 AAH06550 AAH07435 AAH11441 AAH12942
PIR	S41766
REF	NP_001020834 NP_001073196 NP_001100890 NP_001128678 NP_001156008
SP	A5A6M3 D4AE41 P38159 P84586 Q4R7F0
TPG	DAA13328 DAA13329
AlphaFold	Q4R7F0 A5A6M3 D4AE41 P38159 P84586