BMRB Entry 19559

Title:
Backbone resonance assignments of Bcl-2/xL
Deposition date:
2013-10-15
Original release date:
2014-02-12
Authors:
Schwarten, Melanie; Ma, Peixiang; Stoldt, Matthias; Willbold, Dieter
Citation:

Citation: Ma, Peixiang; Schwarten, Melanie; Schneider, Lars; Boeske, Alexandra; Henke, Nadine; Lisak, Dmitrij; Weber, Stephan; Mohrluder, Jeannine; Stoldt, Matthias; Strodel, Birgit; Methner, Axel; Hoffmann, Silke; Weiergraber, Oliver; Willbold, Dieter. "Interaction of Bcl-2 with the Autophagy-related GABAA Receptor-associated Protein (GABARAP): BIOPHYSICAL CHARACTERIZATION AND FUNCTIONAL IMPLICATIONS."  J. Biol. Chem. 288, 37204-37215 (2013).
PubMed: 24240096

Assembly members:

Assembly members:
Bcl-2_xL, polymer, 167 residues, 19387.5379 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts126
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bcl-2/xL1

Entities:

Entity 1, Bcl-2/xL 167 residues - 19387.5379 Da.

1   GLYMETALAHISALAGLYARGTHRGLYTYR
2   ASPASNARGGLUILEVALMETLYSTYRILE
3   HISTYRLYSLEUSERGLNARGGLYTYRGLU
4   TRPASPALAGLYASPASPVALGLUGLUASN
5   ARGTHRGLUALAPROGLUGLYTHRGLUSER
6   GLUVALVALHISLEUTHRLEUARGGLNALA
7   GLYASPASPPHESERARGARGTYRARGARG
8   ASPPHEALAGLUMETSERSERGLNLEUHIS
9   LEUTHRPROPHETHRALAARGGLYARGPHE
10   ALATHRVALVALGLUGLULEUPHEARGASP
11   GLYVALASNTRPGLYARGILEVALALAPHE
12   PHEGLUPHEGLYGLYVALMETCYSVALGLU
13   SERVALASNARGGLUMETSERPROLEUVAL
14   ASPASNILEALALEUTRPMETTHRGLUTYR
15   LEUASNARGHISLEUHISTHRTRPILEGLN
16   ASPASNGLYGLYTRPASPALAPHEVALGLU
17   LEUTYRGLYPROSERMETARG

Samples:

13C-15N_Bcl2_xL: Bcl-2/xL, [U-13C; U-15N], 0.3 mM; Tris-HCl 20.0 mM; DTT 5.0 mM

sample_conditions_1: ionic strength: 20.000 mM; pH: 7.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC13C-15N_Bcl2_xLisotropicsample_conditions_1
3D HNCA13C-15N_Bcl2_xLisotropicsample_conditions_1
CBCACONH (H[N[co[{CA|ca[C]}]]])13C-15N_Bcl2_xLisotropicsample_conditions_1

Software:

ANALYSIS v2.3, CCPN - Assignment, Data evaluation

NMRPipe v7.9.2013.021.23.09, NIH - Conversion, Processing

VnmrJ vdifferent ones, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

  • Varian VNMRS 900 MHz
  • Varian VNMRS 800 MHz
  • Varian VNMRS 600 MHz

Related Database Links:

UniProt P10415
PDB
DBJ BAC81344
GB AAY60777 ABS57368 AET11880 EFB24546 ELR45759
REF XP_003734715 XP_006756883 XP_010634580
AlphaFold Q16197

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks