BMRB Entry 19576

Title:
Backbone resonance assignment of FAPP1 PH domain in the presence of 10% (w/v) DMPC/DHPC (q=0.25), and in the presence of 10% (w/v) DMPC/DHPC (q=0.25) plus 8mM PI4P
Deposition date:
2013-10-23
Original release date:
2014-02-11
Authors:
Prestegard, James; Liu, Yizhou
Citation:

Citation: Liu, Yizhou; Kahn, Richard; Prestegard, James. "Interaction of Fapp1 with Arf1 and PI4P at a membrane surface: an example of coincidence detection."  Structure 22, 421-430 (2014).
PubMed: 24462251

Assembly members:

Assembly members:
FAPP1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20(b)

Data sets:
Data typeCount
13C chemical shifts449
15N chemical shifts191
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FAPP1 PH domain1

Entities:

Entity 1, FAPP1 PH domain 106 residues - Formula weight is not available

1   METGLUGLYVALLEUTYRLYSTRPTHRASN
2   TYRLEUTHRGLYTRPGLNPROARGTRPPHE
3   VALLEUASPASNGLYILELEUSERTYRTYR
4   ASPSERGLNASPASPVALCYSLYSGLYSER
5   LYSGLYSERILELYSMETALAVALCYSGLU
6   ILELYSVALHISSERALAASPASNTHRARG
7   METGLULEUILEILEPROGLYGLUGLNHIS
8   PHETYRMETLYSALAVALASNALAALAGLU
9   ARGGLNARGTRPLEUVALALALEUGLYSER
10   SERLYSALACYSLEUTHRASPTHRLEUGLU
11   HISHISHISHISHISHIS

Samples:

sample_1: FAPP1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DMPC/DHPC 10 % (w/v); K2HPO4-KH2PO4 10 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 5 mM

sample_2: FAPP1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DMPC/DHPC 10 % (w/v); Pi4P 8 mM; K2HPO4-KH2PO4 10 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 900 MHz

Related Database Links:

BMRB 16082 19508
PDB
DBJ BAG37067
GB AAG15199 AAG15200 AAH31110 AAH44567 AAH83759
REF NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546
SP Q9ERS4 Q9HB20
TPG DAA32821
AlphaFold Q9ERS4 Q9HB20

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks