BMRB Entry 19584

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Dvl-2 DEP domain
Published: 2014-04-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19584

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Dvl-2 DEP domain

Deposition date:

2013-10-25

Original release date:

2014-04-14

Authors:

Capelluto, Daniel

Citation:

Citation: Capelluto, Daniel; Zhao, Xiaolin; Lucas, Andrew; Lemkul, Justin; Xiao, Shuyan; Fu, Xiangping; Sun, Furong; Bevan, David; Finkielstein, Carla. "Biophysical and Molecular-Dynamics Studies of Phosphatidic Acid Binding by the Dvl-2 DEP Domain." Biophys. J. 106, 1101-1111 (2014).
PubMed: 24606934

Assembly members:

Assembly members:
Dvl-2_DEP, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dvl-2_DEP: GPLGSCEGRGLSVHMDMASV TKAMAAPESGLEVRDRMWLK ITIPNAFLGSDVVDWLYHHV EGFPERREARKYASGLLKAG LIRHTVNKITFSEQCYYVFG DLSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	274
15N chemical shifts	92
1H chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dvl-2 DEP	1

Entities:

Entity 1, Dvl-2 DEP 104 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

CYS

GLU

GLY

ARG

GLY

2

LEU

SER

VAL

HIS

MET

ASP

MET

ALA

SER

VAL

3

THR

LYS

ALA

MET

ALA

PRO

GLU

SER

GLY

4

LEU

GLU

VAL

ARG

ASP

ARG

MET

TRP

LEU

LYS

5

ILE

THR

ILE

PRO

ASN

ALA

PHE

LEU

GLY

SER

6

ASP

VAL

ASP

TRP

LEU

TYR

HIS

VAL

7

GLU

GLY

PHE

PRO

GLU

ARG

GLU

ALA

ARG

8

LYS

TYR

ALA

SER

GLY

LEU

LYS

ALA

GLY

9

LEU

ILE

ARG

HIS

THR

VAL

ASN

LYS

ILE

THR

10

PHE

SER

GLU

GLN

CYS

TYR

VAL

PHE

GLY

11

ASP

LEU

SER

GLY

Samples:

sample_1: Dvl-2 DEP, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 100 mM; sodium azide 1 mM; DTT-d10, [U-98% 2H], 1 mM; sodium citrate-d4, [U-98% 2H], 20 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz

PDB	3ML6
DBJ	BAE27460 BAE35461 BAE40307 BAG59612 BAG65098
GB	AAB65243 AAC52827 AAH14844 AAH53050 AAH92396
REF	NP_001165527 NP_001178311 NP_001181735 NP_004413 NP_031914
SP	O14641 Q60838
TPG	DAA18923
AlphaFold	Q60838 O14641