BMRB Entry 19593

Title:
Truncated EGF-A
Deposition date:
2013-10-30
Original release date:
2014-02-11
Authors:
Schroeder, Christina; Rosengren, K. Johan
Citation:

Citation: Schroeder, Christina; Swedberg, Joakim; Withka, Jane; Rosengren, K. Johan; Akcan, Muharrem; Clayton, Daniel; Daly, Norelle; Cheneval, Olivier; Borzilleri, Kris; Griffor, Matt; Stock, Ingrid; Colless, Barbara; Walsh, Phillip; Sunderland, Philip; Reyes, Allan; Dullea, Robert; Ammirati, Mark; Liu, Shenping; McClure, Kim; Tu, Meihua; Bhattacharya, Samit; Liras, Spiros; Price, David; Craik, David. "Design and synthesis of truncated EGF-A peptides that restore LDL-R recycling in the presence of PCSK9 in vitro."  Chem. Biol. 21, 284-294 (2014).
PubMed: 24440079

Assembly members:

Assembly members:
egfa, polymer, 27 residues, 2774.056 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
egfa: GTNECLDNNGGCSHVCNDLK IGYECLX

Data sets:
Data typeCount
13C chemical shifts62
1H chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Truncated EGF-A1
2CALCIUM ION2

Entities:

Entity 1, Truncated EGF-A 27 residues - 2774.056 Da.

1   GLYTHRASNGLUCYSLEUASPASNASNGLY
2   GLYCYSSERHISVALCYSASNASPLEULYS
3   ILEGLYTYRGLUCYSLEUNH2

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: egfa 1 mM; d-Tris 50 mM; NaCl 100 mM; CaCl2 5 mM

sample_conditions_1: pH: 5.3; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

Molmol, Koradi, Billeter and Wuthrich - visualisation

TOPSPIN, Bruker Biospin - collection, processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAD92646 BAG10794 BAG58495 BAG59010 BAG61112
GB AAA56833 AAF24515 AAH14514 AAM56036 AAP36025
REF NP_000518 NP_001182727 NP_001182728 NP_001182729 NP_001182732
SP P01130
AlphaFold P01130