BMRB Entry 19669

Name: Backbone 1H, 15N, and 13C resonance assignments of the N-terminal carbohydrate-binding domain of beta-glucan recognition protein 2 from Manduca sexta
Published: 2014-01-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19669

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, and 13C resonance assignments of the N-terminal carbohydrate-binding domain of beta-glucan recognition protein 2 from Manduca sexta

Deposition date:

2013-12-09

Original release date:

2014-01-13

Authors:

Takahashi, Daisuke; Dai, Huaien; Hiromasa, Yasuaki; Krishnamoorthi, Ramaswamy; Kanost, Michael

Citation:

Citation: Takahashi, Daisuke; Dai, Huaien; Hiromasa, Yasuaki; Krishnamoorthi, Ramaswamy; Kanost, Michael. "Backbone 1H, 15N, and 13C resonance assignments of the N-terminal carbohydrate-binding domain of beta-glucan recognition protein 2 from Manduca sexta" .

Assembly members:

Assembly members:
Ms_N-bGRP2, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: tobacco hornworm Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca sexta

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-NbGRP2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ms_N-bGRP2: MGQRGGPYKVPDAKLEAIYP KGLRVSVPDDGYSLFAFHGK LNEEMEGLEAGHWSRDITKA KQGRWIFRDRNAELKLGDKI YFWTYVIKDGLGYRQDNGEW TVTEFVNENGTLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	190
15N chemical shifts	103
1H chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ms-NbGRP2	1

Entities:

Entity 1, Ms-NbGRP2 119 residues - Formula weight is not available

1

MET

GLY

GLN

ARG

GLY

PRO

TYR

LYS

VAL

2

PRO

ASP

ALA

LYS

LEU

GLU

ALA

ILE

TYR

PRO

3

LYS

GLY

LEU

ARG

VAL

SER

VAL

PRO

ASP

4

GLY

TYR

SER

LEU

PHE

ALA

PHE

HIS

GLY

LYS

5

LEU

ASN

GLU

MET

GLU

GLY

LEU

GLU

ALA

6

GLY

HIS

TRP

SER

ARG

ASP

ILE

THR

LYS

ALA

7

LYS

GLN

GLY

ARG

TRP

ILE

PHE

ARG

ASP

ARG

8

ASN

ALA

GLU

LEU

LYS

LEU

GLY

ASP

LYS

ILE

9

TYR

PHE

TRP

THR

TYR

VAL

ILE

LYS

ASP

GLY

10

LEU

GLY

TYR

ARG

GLN

ASP

ASN

GLY

GLU

TRP

11

THR

VAL

THR

GLU

PHE

VAL

ASN

GLU

ASN

GLY

12

THR

LEU

GLU

HIS

Samples:

sample_1: Ms N-bGRP2, [U-99% 13C; U-99% 15N], 1.5 mM; sodium phosphate 20 mM; sodium azide 3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v3.2, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Wuthrich group, ETH Zurich - chemical shift assignment, data analysis

NMR spectrometers:

Varian VNMR 500 MHz

GB	AAN10151
SP	Q8ISB6
AlphaFold	Q8ISB6