BMRB Entry 19767

Title:
Solid-state NMR assignment of metal-free SOD1 fibrillar structures
Deposition date:
2014-02-05
Original release date:
2014-06-05
Authors:
Banci, Lucia; Blazevits, Olga; Cantini, Francesca; Danielsson, Jens; Lang, Lisa; Luchinat, Claudio; Mao, Jiafei; Oliveberg, Mikael; Ravera, Enrico
Citation:

Citation: Banci, Lucia; Blaevit, Olga; Cantini, Francesca; Danielsson, Jens; Lang, Lisa; Luchinat, Claudio; Mao, Jiafei; Oliveberg, Mikael; Ravera, Enrico. "Solid-state NMR studies of metal-free SOD1 fibrillar structures."  J. Biol. Inorg. Chem. 19, 659-666 (2014).
PubMed: 24719206

Assembly members:

Assembly members:
apoSOD1-DeltaIV-VII, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoSOD1-DeltaIV-VII1

Entities:

Entity 1, apoSOD1-DeltaIV-VII 110 residues - Formula weight is not available

1   ALATHRLYSALAVALALAVALLEULYSGLY
2   ASPGLYPROVALGLNGLYILEILEASNPHE
3   GLUGLNLYSGLUSERASNGLYPROVALLYS
4   VALTRPGLYSERILELYSGLYLEUTHRGLU
5   GLYLEUHISGLYPHEHISVALHISGLYALA
6   GLYGLYASPLEUGLYASNVALTHRALAASP
7   LYSASPGLYVALALAASPVALSERILEGLU
8   ASPSERVALILESERLEUSERGLYASPHIS
9   SERILEILEGLYARGTHRLEUVALVALHIS
10   GLULYSALAGLYALAGLYALAGLYSERARG
11   LEUALASERGLYVALILEGLYILEALAGLN

Samples:

sample_1: apoSOD1-DeltaIV-VII, [U-13C; U-15N], na na

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1solidsample_conditions_1
2D NCAsample_1solidsample_conditions_1
2D NCOsample_1solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
1H-13C INEPT-HETCORsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB