BMRB Entry 19922

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19922

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of human TDP-43 RRM2
Deposition date:
2014-04-18
Original release date:
2014-05-02
Authors:
Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai
Citation:

Citation: Rabdano, Sevastyan; Podkorytov, Ivan; Yuwen, Tairan; Groves, Adam; Chamberlain, Kyle; Yakimov, Alexander; Skrynnikov, Nikolai. "Backbone 1H, 13C, and 15N Chemical Shift Assignments of human TDP-43 RRM2"  .

Assembly members:

Assembly members:
TDP-43_RRM2, polymer, 76 residues, 8703 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TDP-43_RRM2: MRKVFVGRCTEDMTEDELRE FFSQYGDVMDVFIPKPFRAF AFVTFADDQIAQSLCGEDLI IKGISVHISNAEPKHN

Data sets:
Data typeCount
13C chemical shifts221
1H chemical shifts69
15N chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TDP-43 RRM2, 11
2TDP-43 RRM2, 21

Entities:

Entity 1, TDP-43 RRM2, 1 76 residues - 8703 Da.

1   METARGLYSVALPHEVALGLYARGCYSTHR
2   GLUASPMETTHRGLUASPGLULEUARGGLU
3   PHEPHESERGLNTYRGLYASPVALMETASP
4   VALPHEILEPROLYSPROPHEARGALAPHE
5   ALAPHEVALTHRPHEALAASPASPGLNILE
6   ALAGLNSERLEUCYSGLYGLUASPLEUILE
7   ILELYSGLYILESERVALHISILESERASN
8   ALAGLUPROLYSHISASN

Samples:

sample_1: TDP-43 RRM2, [U-13C; U-15N], 1.5 mM; H2O 90%; D2O, [U-2H], 10%; sodium phosphate 20 mM; DTT 22 mM; sodium azide 0.2%

sample_conditions_1: temperature: 298 K; pH: 6.7; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection, processing

CARA v1.9.0.0, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance-III 500 MHz

Related Database Links:

BMRB 19290
PDB
DBJ BAD96474 BAE87159 BAG35326 BAG58707 BAG61425
EMBL CAB43367 CAG38565 CAH92854 CAL37794
GB AAA70033 AAH71657 AAH95435 ABO32290 ABO32291
REF NP_001127597 NP_031401 XP_001102660 XP_002750326 XP_003274288
SP Q13148 Q5R5W2
AlphaFold Q5R5W2 Q13148

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks