BMRB Entry 19929

Title:
HP24stab derived from the villin headpiece subdomain
Deposition date:
2014-04-23
Original release date:
2015-02-16
Authors:
Hocking, Henry; Haese, F.; Madl, T.; Zacharias, M.; Rief, M.; Zoldak, G.
Citation:

Citation: Hocking, Henry; Haese, F.; Madl, T.; Zacharias, M.; Rief, M.; Zoldak, G.. "A new minimal native-like compact supersecondary substructure HP24stab derived from the villin headpiece subdomain"  To Be Published ., .-..

Assembly members:

Assembly members:
VILLIN-1, polymer, 26 residues, 2853.4511 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VILLIN-1: XMTRSAFANLPLWKQQALMK EKGLFX

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts24
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VILLIN-11

Entities:

Entity 1, VILLIN-1 26 residues - 2853.4511 Da.

1   ACEMETTHRARGSERALAPHEALAASNLEU
2   PROLEUTRPLYSGLNGLNALALEUMETLYS
3   GLULYSGLYLEUPHENH2

Samples:

sample_1: VILLIN-1, [U-13C; U-15N], 3 mM

sample_conditions_1: ionic strength: 50.000 mM; pH: 7.500; pressure: 1 atm; temperature: 288.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

UNP VILI_CHICK
PDB
AlphaFold P02640

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks