BMRB Entry 19933

Title:
Membrane induced structure of novel human tachykinin Hemokinin-1 (hHK1)
Deposition date:
2014-04-24
Original release date:
2015-05-26
Authors:
Ganjiwale, Anjali; Cowsik, Sudha M
Citation:

Citation: Ganjiwale, Anjali; Cowsik, Sudha M. "Membrane induced structure of novel human tachykinin Hemokinin-1"  Biopolymers ., .-..

Assembly members:

Assembly members:
entity, polymer, 11 residues, 2356.776 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: TGKASQFFGLM

Data sets:
Data typeCount
1H chemical shifts50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 11 residues - 2356.776 Da.

1   THRGLYLYSALASERGLNPHEPHEGLYLEU
2   MET

Samples:

sample_1: Hemokinin 4.5 mM; H2O 90%; D2O, [U-2H], 10%; SDS, [U-99% 2H], 300 mM

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - data analysis, refinement, structure solution

TOPSPIN, Bruker Biospin - chemical shift calculation, processing

NMR spectrometers:

  • Bruker Avance 700 MHz