BMRB Entry 19992

Title:
Kelch domain of human Keap1
Deposition date:
2014-05-28
Original release date:
2015-03-11
Authors:
Khan, Halema; Choy, Wing Yiu; Killoran, Ryan; Fan, Jingsong; Yang, Daiwen; Brickenden, Anne
Citation:

Citation: Khan, Halema; Killoran, Ryan; Brickenden, Anne; Fan, Jingsong; Yang, Daiwen; Choy, Wing-Yiu. "Molecular effects of cancer-associated somatic mutations on the structural and target recognition properties of Keap1"  Biochem. J. 467, 139-149 (2015).
PubMed: 25582950

Assembly members:

Assembly members:
human_Kelch_domain, polymer, 293 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
1H chemical shifts252
15N chemical shifts252

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kelch domain1

Entities:

Entity 1, Kelch domain 293 residues - Formula weight is not available

1   GLYSERHISMETALAPROLYSVALGLYARG
2   LEUILETYRTHRALAGLYGLYTYRPHEARG
3   GLNSERLEUSERTYRLEUGLUALATYRASN
4   PROSERASPGLYTHRTRPLEUARGLEUALA
5   ASPLEUGLNVALPROARGSERGLYLEUALA
6   GLYCYSVALVALGLYGLYLEULEUTYRALA
7   VALGLYGLYARGASNASNSERPROASPGLY
8   ASNTHRASPSERSERALALEUASPCYSTYR
9   ASNPROMETTHRASNGLNTRPSERPROCYS
10   ALAPROMETSERVALPROARGASNARGILE
11   GLYVALGLYVALILEASPGLYHISILETYR
12   ALAVALGLYGLYSERHISGLYCYSILEHIS
13   HISASNSERVALGLUARGTYRGLUPROGLU
14   ARGASPGLUTRPHISLEUVALALAPROMET
15   LEUTHRARGARGILEGLYVALGLYVALALA
16   VALLEUASNARGLEULEUTYRALAVALGLY
17   GLYPHEASPGLYTHRASNARGLEUASNSER
18   ALAGLUCYSTYRTYRPROGLUARGASNGLU
19   TRPARGMETILETHRALAMETASNTHRILE
20   ARGSERGLYALAGLYVALCYSVALLEUHIS
21   ASNCYSILETYRALAALAGLYGLYTYRASP
22   GLYGLNASPGLNLEUASNSERVALGLUARG
23   TYRASPVALGLUTHRGLUTHRTRPTHRPHE
24   VALALAPROMETLYSHISARGARGSERALA
25   LEUGLYILETHRVALHISGLNGLYARGILE
26   TYRVALLEUGLYGLYTYRASPGLYHISTHR
27   PHELEUASPSERVALGLUCYSTYRASPPRO
28   ASPTHRASPTHRTRPSERGLUVALTHRARG
29   METTHRSERGLYARGSERGLYVALGLYVAL
30   ALAVALTHR

Samples:

15N_Kelch: human Kelch domain, [U-100% 13C; U-100% 15N; U-80% 2H], 600 uM; Sodium Phosphate 50 mM; NaCl 100 mM; D2O 10%; H2O 90%

sample_conditions_1: temperature: 273 K; pH: 7.0; pressure: 1 atm; ionic strength: 150 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_Kelchisotropicsample_conditions_1
3D CBCA(CO)NH15N_Kelchisotropicsample_conditions_1
3D HNCACB15N_Kelchisotropicsample_conditions_1
3D 1H-15N NOESY15N_Kelchisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA09481 BAG09615 BAG51647 BAK62444
EMBL CAG15151 CAH92386
GB AAH02417 AAH02930 AAH15945 AAI51546 AAK43722
REF NP_001094612 NP_001108143 NP_001126406 NP_001266890 NP_036421
SP Q14145 Q5R774 Q684M4
TPG DAA27941
AlphaFold Q14145 Q684M4 Q5R774

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks