BMRB Entry 21062

Title:
conotoxin Im10A
Deposition date:
2016-01-24
Original release date:
2016-05-24
Authors:
Jiang, Ling
Citation:

Citation: Yua, Shuo; Du, Tianpeng; Liu, Zhuguo; Wu, Qiaoling; Feng, Guixue; Dong, Mingxin; Zhou, Xiaowei; Jiang, Ling; Dai, Qiuyun. "Im10A, a short conopeptide isolated from Conus imperialis and possesses two highly concentrated disulfide bridges and analgesic activity."  Peptides 81, 15-20 (2016).
PubMed: 27131596

Assembly members:

Assembly members:
Im10A, polymer, 11 residues, 1239.472 Da.

Natural source:

Natural source:   Common Name: Conus imperialis   Taxonomy ID: 35631   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus imperialis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Im10A: NTICCEGCMCY

Data sets:
Data typeCount
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Im10A1

Entities:

Entity 1, Im10A 11 residues - 1239.472 Da.

1   ASNTHRILECYSCYSGLUGLYCYSMETCYS
2   TYR

Samples:

sample_1: entity0.3 – 0.5 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.5.5, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz