BMRB Entry 25023

Title:
15N HSQC assignment of Drosophila ELF domain from FANCL
Deposition date:
2014-06-16
Original release date:
2016-07-01
Authors:
Miles, Jennifer; Carroll, Ellis; Rowe, Michelle; Howard, Mark; Sihu, Ateesh; Frost, Mark; Williamson, Richard; Alpi, Arno; Walden, Helen
Citation:

Citation: Miles, Jennifer; Carroll, Ellis; Rowe, Michelle; Howard, Mark; Sidhu, Ateesh; Frost, Mark; Williamson, Richard; Alpi, Arno; Walden, Helen. "The Fanconi Anemia DNA Repair Pathway Is Regulated by an Interaction between Ubiquitin and the E2-like Fold Domain of FANCL"  J. Biol. Chem. 290, 20995-21006 (2015).
PubMed: 26149689

Assembly members:

Assembly members:
ELF, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a SUMO

Data sets:
Data typeCount
15N chemical shifts74
1H chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ELF1

Entities:

Entity 1, ELF 104 residues - Formula weight is not available

1   METGLUSERASNGLUASPVALGLUARGLEU
2   LEUCYSGLNLYSTYRPROGLYLEUALAALA
3   GLULEUGLNPROSERGLYALACYSILEILE
4   ARGGLYVALLEUGLYSERGLUASPTHRTRP
5   ARGARGLEULYSLEUTYRLEUPROHISHIS
6   PROALALEUHISGLYPHEGLNLEUTYRVAL
7   GLNGLUSERLEUGLUTYRLYSLEUTYRTHR
8   SERALAASNLEULYSLEUGLNASPASPTRP
9   LEULEUGLUASPPHELEUASPHISLEUPRO
10   LYSILELEUPROALAGLNLYSALAPROTHR
11   VALPROLYSGLU

Samples:

sample_1: ELF, [U-99% 15N], 0.6 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Unity 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks