BMRB Entry 25046

Title:
Backbone 1H, 15N Chemical Shift Assignments of the DC-SIGNR carbohydrate recognition domain in the Apo state.
Deposition date:
2014-06-25
Original release date:
2014-07-25
Authors:
Probert, Fay; Dixon, Ann; Mitchell, Dan
Citation:

Citation: Probert, Fay; Dixon, Ann; Mitchell, Dan. "NMR evidence for oligosaccharide release from the DC-SIGNR (CLEC4M) carbohydrate recognition domain at low pH"  FEBS J. ., .-..

Assembly members:

Assembly members:
DC-SIGNR, polymer, 139 residues, 16194.7 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT5T

Data sets:
Data typeCount
1H chemical shifts84
15N chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DC-SIGNR CRD1

Entities:

Entity 1, DC-SIGNR CRD 139 residues - 16194.7 Da.

1   ALAGLUARGLEUCYSARGHISCYSPROLYS
2   ASPTRPTHRPHEPHEGLNGLYASNCYSTYR
3   PHEMETSERASNSERGLNARGASNTRPHIS
4   ASPSERVALTHRALACYSGLNGLUVALARG
5   ALAGLNLEUVALVALILELYSTHRALAGLU
6   GLUGLNASNPHELEUGLNLEUGLNTHRSER
7   ARGSERASNARGPHESERTRPMETGLYLEU
8   SERASPLEUASNGLNGLUGLYTHRTRPGLN
9   TRPVALASPGLYSERPROLEUSERPROSER
10   PHEGLNARGTYRTRPASNSERGLYGLUPRO
11   ASNASNSERGLYASNGLUASPCYSALAGLU
12   PHESERGLYSERGLYTRPASNASPASNARG
13   CYSASPVALASPASNTYRTRPILECYSLYS
14   LYSPROALAALACYSPHEARGASPGLU

Samples:

sample_1: DC-SIGNR, [U-100% 15N], 0.7 mM; HEPES, [U-100% 2H], 20 mM; NaCl 20 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 310 K; pH: 6.8; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

NCBI 9606
BMRB 19297
PDB
DBJ BAF84967 BAG65307
GB AAG13815 AAG13848 AAH38851 AAI10615 AAK20998
REF NP_001138376 NP_001138377 NP_001138378 NP_001138379 NP_001138381
SP Q8HY06 Q9H2X3
AlphaFold Q8HY06 Q9H2X3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks