BMRB Entry 25119

Title:
Chemical shift assignments for human cardiac troponin I, residues 1-73, bound to cardiac troponin C
Deposition date:
2014-07-31
Original release date:
2014-09-03
Authors:
Hwang, Peter
Citation:

Citation: Hwang, Peter. "cTnI[1-73] bound to cTnC"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
cTnI[1-73], polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-PagP

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts434
13C chemical shifts282
15N chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cTnI[1-73]-cTnC1

Entities:

Entity 1, cTnI[1-73]-cTnC 80 residues - Formula weight is not available

1   LEUALAASPGLYSERSERASPALAALAARG
2   GLUPROARGPROALAPROALAPROILEARG
3   ARGARGSERSERASNTYRARGALATYRALA
4   THRGLUPROHISALALYSLYSLYSSERLYS
5   ILESERALASERARGLYSLEUGLNLEULYS
6   THRLEULEULEUGLNILEALALYSGLNGLU
7   LEUGLUARGGLUALAGLUGLUARGARGGLY
8   GLULYSGLYARGHISHISHISHISHISHIS

Samples:

sample_1: cTnI[1-73], [U-100% 13C; U-100% 15N], 0.5 mM; cTnC 0.5 mM; H20 95%; D20 5%

sample_conditions_1: temperature: 313 K; pH: 6.2; pressure: 1 atm; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 25118
DBJ BAD97171
EMBL CAA38102 CAA62301 CAG46782 CAG46809
GB AAA16157 AAH96165 AAH96166 AAH96167 AAH99631
PRF 1616225A
REF NP_000354 XP_003807165 XP_004061509 XP_009434690
SP P19429
AlphaFold P19429

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks