BMRB Entry 25168

Title:
flpp3sol BMRB submission
Deposition date:
2014-08-20
Original release date:
2015-10-23
Authors:
Zook, James; Mo, Gina; Craciunescu, Felicia; Sisco, Nick; Hansen, Debra; Baravati, Bobby; Van Horn, Wade; Cherry, Brian; Fromme, Petra
Citation:

Citation: Zook, James; Mo, Gina; Craciunescu, Felicia; Sisco, Nick; Hansen, Debra; Baravati, Bobby; Cherry, Brian; Van Horn, Wade; Fromme, Petra. "NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins"  Structure 23, 1116-1122 (2015).
PubMed: 26004443

Assembly members:

Assembly members:
flpp3Sol_2, polymer, 119 residues, 13265.504 Da.

Natural source:

Natural source:   Common Name: Francisella tularensis   Taxonomy ID: 177416   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Francisella tularensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Data typeCount
13C chemical shifts421
15N chemical shifts112
1H chemical shifts646

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1flpp3Sol 21

Entities:

Entity 1, flpp3Sol 2 119 residues - 13265.504 Da.

1   METASPLYSGLYTHRGLNTYRLYSASPGLY
2   TYRTYRILETHRTHRLEUASNTYRASNPHE
3   ASNTHRVALTYRASNALATHRLEUGLNALA
4   ILEGLNASNGLYGLNTHRPHEASPTYRLYS
5   SERASNPROTYRASPILESERVALASNLYS
6   ASNASNGLYTHRASPALAGLUILEVALSER
7   ALASERASPSERASPSERTHRASPSERLEU
8   GLNVALALAMETLYSLYSLEUPROASNASN
9   ALATHRARGILESERILELYSTYRGLYSER
10   GLNGLYASNSERILEARGSERSERALALEU
11   ILEGLYILEILEGLUGLYASNILEARGTYR
12   ALAASNTHRHISHISHISHISHISHIS

Samples:

N-flpp3sol: flpp3Sol_2, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 95%; D2O 5%; Sodium Chloride 90 mM; Sodium Phosphate 20 mM

NC-flpp3sol: flpp3Sol_2, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 95%; D2O 5%; Sodium Chloride 90 mM; Sodium Phosphate 20 mM

Sample: ionic strength: 0.110 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.150 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCN-flpp3solisotropicSample
3D HNCONC-flpp3solisotropicSample
HNcaCO (HNcaCO)NC-flpp3solisotropicSample
3D HNCANC-flpp3solisotropicSample
3D HNCACBNC-flpp3solisotropicSample
3D CBCA(CO)NHNC-flpp3solisotropicSample
3D 1H-15N NOESYNC-flpp3solisotropicSample
CcoNH (C_coNH.relayed)NC-flpp3solisotropicSample
3D HCCH-TOCSYNC-flpp3solisotropicSample
3D H(CCO)NHNC-flpp3solisotropicSample
3D 1H-13C NOESYNC-flpp3solisotropicSample

Software:

CcpNmr_Analysis v2.4, CCPN - Spectrum analysis

nmrDraw vany, Delaglio - Spectrum display

nmrPipe vany, Delaglio - Spectrum processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Related Database Links:

PDB
EMBL CAG46049 CAJ79085 CAL09432
GB AAX78073 ABI82600 ABK90252 ABO46387 ABU61155
REF WP_003015055 WP_003018179 WP_003034620 WP_003037248 WP_012429332

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks